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SthK closed state, cAMP-bound in the presence of POPA

Electron Microscopy
2.41Å resolution
PDB entry 7tj5 coloured by chain, front view.
PDB entry 7tj5 coloured by chain, side view.
PDB entry 7tj5 coloured by chain, top view.
Source organism: Spirochaeta thermophila DSM 6578
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Cyclic nucleotide-binding domain-containing protein
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Multimeric state: homo tetramer
Accessible surface area: 75371.36 Å2
Buried surface area: 24621.4 Å2
Dissociation area: 11,206.21 Å2
Dissociation energy (ΔGdiss): 114.08 kcal/mol
Dissociation entropy (TΔSdiss): 48.18 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-123836

Macromolecules

Cyclic nucleotide-binding domain-containing protein
Chains: A, B, C, D
Length: 456 amino acids
Theoretical weight: 51.12 KDa
Source organism: Spirochaeta thermophila DSM 6578
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0GA88 (Residues: 1-420; Coverage: 98%)
Gene name: Spith_0644
Pfam:
InterPro:
Cyclic nucleotide-binding domain-containing protein in PDB entry 7tj5, assembly 1, front view.
Cyclic nucleotide-binding domain-containing protein in PDB entry 7tj5, assembly 1, side view.
Cyclic nucleotide-binding domain-containing protein in PDB entry 7tj5, assembly 1, top view.
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