Sample information
Author description:
Structure of the rabbit 80S ribosome stalled on a 2-TMD Rhodopsin intermediate in complex with the multipass translocon
Source organisms:
Validation information
Experimental information
Specimen preparation
| Buffer name | pH | Details |
|---|---|---|
| - | 7.5 | 50 mM HEPES-KOH, pH 7.5, 200 mM KOAc, 5 mM Mg(OAc)2, 0.25% digitonin, 50 mM biotin |
Vitrification
| Cryogen | Temperature | Instrument | Humidity | Details |
|---|---|---|---|---|
| - | - | FEI VITROBOT MARK IV | 100 | blot for 4 sec with Whatman filter papers at a blot force of -15 before plunging |
Refinement statistics
Structure solution method:
Electron Microscopy
Resolution:
3.25 Å
