› 7tqa

X-ray diffraction
2.33Å resolution

Crystal Structure of monoclonal S9.6 Fab

Released:
DOI: 10.2210/pdb7tqa/pdb
PDB entry 7tqa coloured by chain, front view.
PDB entry 7tqa coloured by chain, side view.
PDB entry 7tqa coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structural basis of R-loop recognition by the S9.6 monoclonal antibody.
Bou-Nader C, Bothra A, Garboczi DN, Leppla SH, Zhang J
OpenAccess logo Nat Commun 13 1641 (2022)
PMID: 35347133

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-214473 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab S9.6 heavy chain Chains: A, C, H
Molecule details ›
Chains: A, C, H
Length: 231 amino acids
Theoretical weight: 24.97 KDa
Source organism: synthetic construct
Expression system: Cricetulus griseus
Fab S9.6 light chain Chains: B, D, L
Molecule details ›
Chains: B, D, L
Length: 219 amino acids
Theoretical weight: 24.12 KDa
Source organism: synthetic construct
Expression system: Cricetulus griseus

Ligands and Environments

2 bound ligands:
Ligand SO4 12 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P31
Unit cell:
a: 96.649Å b: 96.649Å c: 140.583Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.179 0.235
Expression system: Cricetulus griseus

Quick links

Citations

20 citation in other articles

PARP1 associates with R-loops to promote their resolution and genome stability.
Laspata et al. (2023)
19 more