› 7trg

Electron Microscopy
3Å resolution

The beta-tubulin folding intermediate I

Released:
DOI: 10.2210/pdb7trg/pdb
PDB entry 7trg coloured by chain, front view.
PDB entry 7trg coloured by chain, side view.
PDB entry 7trg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural visualization of the tubulin folding pathway directed by human chaperonin TRiC/CCT.
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell 185 4770-4787.e20 (2022)
PMID: 36493755
Related structures: EMD-26089

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-139587 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Tubulin beta chain Chain: A
Molecule details ›
Chain: A
Length: 444 amino acids
Theoretical weight: 49.72 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P07437 (Residues: 1-444; Coverage: 100%)
Gene names: OK/SW-cl.56, TUBB, TUBB5
Sequence domains:
T-complex protein 1 subunit theta Chain: B
Molecule details ›
Chain: B
Length: 547 amino acids
Theoretical weight: 59.58 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P50990 (Residues: 1-547; Coverage: 100%)
Gene names: C21orf112, CCT8, CCTQ, KIAA0002
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit eta Chain: C
T-complex protein 1 subunit epsilon Chain: D
T-complex protein 1 subunit beta Chain: E
T-complex protein 1 subunit delta Chain: F
T-complex protein 1 subunit alpha Chain: G
T-complex protein 1 subunit gamma Chain: H
T-complex protein 1 subunit zeta Chain: I

Ligands and Environments

3 bound ligands:
Ligand MG 8 x MG
Ligand ADP 8 x ADP
Ligand AF3 8 x AF3
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3Å
Relevant EMDB volumes: EMD-26089
Expression system: Trichoplusia ni

Quick links

Citations

5 review citations

Mechanisms and pathology of protein misfolding and aggregation.
Louros et al. (2023)
4 more

1 mention without citation

Structural visualization of the tubulin folding pathway directed by human chaperonin TRiC/CCT.
Gestaut et al. (2022)