7u4t

Electron Microscopy
3.6Å resolution

Human V-ATPase in state 2 with SidK and mEAK-7

Released:
Primary publication:
Identification of mEAK-7 as a human V-ATPase regulator via cryo-EM data mining.
OpenAccess logo Proc Natl Acad Sci U S A 119 e2203742119 (2022)
PMID: 35994636
Related structures: EMD-26334

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 36-mer (preferred)
PDBe Complex ID:
PDB-CPX-127432 (preferred)
Entry contents:
18 distinct polypeptide molecules
Macromolecules (18 distinct):
V-type proton ATPase 116 kDa subunit a 1 Chain: R
Molecule details ›
Chain: R
Length: 837 amino acids
Theoretical weight: 96.51 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q93050 (Residues: 1-837; Coverage: 100%)
Gene names: ATP6N1, ATP6N1A, ATP6V0A1, VPP1
Sequence domains: V-type ATPase 116kDa subunit family
V-type proton ATPase subunit C 1 Chain: O
Molecule details ›
Chain: O
Length: 382 amino acids
Theoretical weight: 44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P21283 (Residues: 1-382; Coverage: 100%)
Gene names: ATP6C, ATP6D, ATP6V1C1, VATC
Sequence domains: V-ATPase subunit C
V-type proton ATPase subunit E 1 Chains: H, I, J
V-type proton ATPase subunit G 1 Chains: K, L, M
V-type proton ATPase subunit e 1 Chain: S
Ribonuclease kappa Chain: T
V-type proton ATPase subunit S1 Chain: U
Renin receptor Chain: V
V-type proton ATPase 21 kDa proteolipid subunit c'' Chain: 0
V-type proton ATPase 16 kDa proteolipid subunit c Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9
V-type proton ATPase subunit d 1 Chain: Q
V-type proton ATPase catalytic subunit A Chains: A, B, C
V-type proton ATPase subunit B, brain isoform Chains: D, E, F
Type IV secretion protein Dot Chains: X, Y, Z
V-type proton ATPase subunit D Chain: G
V-type proton ATPase subunit F Chain: N
V-type proton ATPase subunit H Chain: P
MTOR-associated protein MEAK7 Chain: W

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-26334
Expression system: Escherichia coli