› 7ua4

Electron Microscopy
2.93Å resolution

Structure of PKA phosphorylated human RyR2-R2474S in the open state in the presence of Calmodulin

Released:
DOI: 10.2210/pdb7ua4/pdb
PDB entry 7ua4 coloured by chain, front view.
PDB entry 7ua4 coloured by chain, side view.
PDB entry 7ua4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analyses of human ryanodine receptor type 2 channels reveal the mechanisms for sudden cardiac death and treatment.
Miotto MC, Weninger G, Dridi H, Yuan Q, Liu Y, Wronska A, Melville Z, Sittenfeld L, Reiken S, Marks AR
OpenAccess logo Sci Adv 8 eabo1272 (2022)
PMID: 35857850
Related structures: EMD-26414

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143994 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ryanodine receptor 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 4967 amino acids
Theoretical weight: 565.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92736 (Residues: 1-4967; Coverage: 100%)
  • Best match: Q92736-2 (Residues: 1-4975)
Gene name: RYR2
Sequence domains:
Peptidyl-prolyl cis-trans isomerase FKBP1B Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 108 amino acids
Theoretical weight: 11.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68106 (Residues: 1-108; Coverage: 100%)
Gene names: FKBP12.6, FKBP1B, FKBP1L, FKBP9, OTK4
Sequence domains: FKBP-type peptidyl-prolyl cis-trans isomerase
Calmodulin-1 Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand ATP 8 x ATP
Ligand CA 4 x CA
Ligand XAN 4 x XAN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.93Å
Relevant EMDB volumes: EMD-26414
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

3 review citations

Three-dimensional perspective on ryanodine receptor mutations causing skeletal and cardiac muscle-related diseases.
Iyer et al. (2023)
2 more

1 mention without citation

Structural analyses of human ryanodine receptor type 2 channels reveal the mechanisms for sudden cardiac death and treatment.
Miotto et al. (2022)