› 7ucg

Electron Microscopy
3.5Å resolution

Structure of the DU422 SOSIP.664 trimer in complex with neutralizing antibody Fab fragments 10-1074 and BG24

Released:
DOI: 10.2210/pdb7ucg/pdb
PDB entry 7ucg coloured by chain, front view.
PDB entry 7ucg coloured by chain, side view.
PDB entry 7ucg coloured by chain, top view.
Source organisms:
Primary publication:
A naturally arising broad and potent CD4-binding site antibody with low somatic mutation.
Barnes CO, Schoofs T, Gnanapragasam PNP, Golijanin J, Huey-Tubman KE, Gruell H, Schommers P, Suh-Toma N, Lee YE, Cetrulo Lorenzi JC, Piechocka-Trocha A, Scheid JF, West AP, Walker BD, Seaman MS, Klein F, Nussenzweig MC, Bjorkman PJ
Sci Adv 8 eabp8155 (2022)
PMID: 35960796
Related structures: EMD-26443

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212088 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (9 distinct):
Transmembrane protein gp41 Chains: A, B, M
Molecule details ›
Chains: A, B, M
Length: 153 amino acids
Theoretical weight: 17.1 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q202J5 (Residues: 509-659; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
BG24 CDRH2-v2 Fab heavy chain Chains: C, D, N
Molecule details ›
Chains: C, D, N
Length: 234 amino acids
Theoretical weight: 25.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
BG24 light chain Chains: E, F, O
Molecule details ›
Chains: E, F, O
Length: 205 amino acids
Theoretical weight: 21.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Envelope glycoprotein gp160 Chains: G, I, P
Molecule details ›
Chains: G, I, P
Length: 507 amino acids
Theoretical weight: 57 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q202J5 (Residues: 28-503; Coverage: 56%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
10-1074 Fab heavy chain Chains: H, J, Q
Molecule details ›
Chains: H, J, Q
Length: 243 amino acids
Theoretical weight: 26.39 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
10-1074 light chain Chains: K, L, R
Molecule details ›
Chains: K, L, R
Length: 216 amino acids
Theoretical weight: 23.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Ligand NAG 42 x NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Ligand NAG 6 x NAG
Ligand BMA 3 x BMA
Ligand MAN 6 x MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Ligand NAG 6 x NAG
Ligand BMA 3 x BMA
Ligand MAN 12 x MAN
1 bound ligand:
Ligand NAG 21 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-26443
Expression system: Homo sapiens

Quick links

Citations

2 review citations

'Immunization during ART and ATI for HIV-1 vaccine discovery/development'.
Stamatatos L. (2023)
1 more