› 7ue9

X-ray diffraction
1.75Å resolution

Structure of anti-C3d Fab(3d8b) in complex with C3d

Released:
DOI: 10.2210/pdb7ue9/pdb
PDB entry 7ue9 coloured by chain, front view.
PDB entry 7ue9 coloured by chain, side view.
PDB entry 7ue9 coloured by chain, top view.
Source organisms:
Primary publication:
Development and Optimization of Bifunctional Fusion Proteins to Locally Modulate Complement Activation in Diseased Tissue.
Fahnoe KC, Liu F, Morgan JG, Ryan ST, Storek M, Stark EG, Taylor FR, Holers VM, Thurman JM, Wawersik S, Kalled SL, Violette SM
OpenAccess logo Front Immunol 13 869725 (2022)
PMID: 35784298

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224930 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 241 amino acids
Theoretical weight: 26.55 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 456 amino acids
Theoretical weight: 49.72 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Complement C3d fragment Chain: C
Molecule details ›
Chain: C
Length: 311 amino acids
Theoretical weight: 34.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01024 (Residues: 994-1303; Coverage: 19%)
Gene names: C3, CPAMD1
Sequence domains: A-macroglobulin TED domain

Ligands and Environments

1 bound ligand:
Ligand GOL 10 x GOL
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 73.372Å b: 62.229Å c: 85.547Å
α: 90° β: 108.03° γ: 90°
R-values:
R R work R free
0.172 0.171 0.201
Expression systems:
  • Cricetulus griseus
  • Escherichia coli

Quick links

Citations

1 review citation

C3-dependent effector functions of complement.
Zarantonello et al. (2023)
2 other articles

1 mention without citation

Development and Optimization of Bifunctional Fusion Proteins to Locally Modulate Complement Activation in Diseased Tissue.
Fahnoe et al. (2022)