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Structure of anti-C3d Fab(3d8b) in complex with C3d

X-ray diffraction
1.75Å resolution
PDB entry 7ue9 coloured by chain, front view.
PDB entry 7ue9 coloured by chain, side view.
PDB entry 7ue9 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Complement C3d fragment and IG-light kappa chain and IG-heavy chain
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Multimeric state: hetero trimer
Accessible surface area: 31566.78 Å2
Buried surface area: 7896.35 Å2
Dissociation area: 1,131.23 Å2
Dissociation energy (ΔGdiss): -2.86 kcal/mol
Dissociation entropy (TΔSdiss): 13.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-224930

Macromolecules

Fab light chain
Chain: L
Length: 241 amino acids
Theoretical weight: 26.55 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
InterPro:
Fab light chain in PDB entry 7ue9, assembly 1, front view.
Fab light chain in PDB entry 7ue9, assembly 1, side view.
Fab light chain in PDB entry 7ue9, assembly 1, top view.
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Fab heavy chain
Chain: H
Length: 456 amino acids
Theoretical weight: 49.72 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
InterPro:
Fab heavy chain in PDB entry 7ue9, assembly 1, front view.
Fab heavy chain in PDB entry 7ue9, assembly 1, side view.
Fab heavy chain in PDB entry 7ue9, assembly 1, top view.
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Complement C3d fragment
Chain: C
Length: 311 amino acids
Theoretical weight: 34.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01024 (Residues: 994-1303; Coverage: 19%)
Gene names: C3, CPAMD1
Pfam: A-macroglobulin TED domain
InterPro:
Complement C3d fragment in PDB entry 7ue9, assembly 1, front view.
Complement C3d fragment in PDB entry 7ue9, assembly 1, side view.
Complement C3d fragment in PDB entry 7ue9, assembly 1, top view.
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