› 7ugp

Electron Microscopy
4.2Å resolution

Cryo-EM structure of BG24 Fabs with an inferred germline light chain and 10-1074 Fabs in complex with HIV-1 Env immunogen BG505-SOSIPv4.1-GT1 containing the N276 gp120 glycan- Class 1

Released:
DOI: 10.2210/pdb7ugp/pdb
PDB entry 7ugp coloured by chain, front view.
PDB entry 7ugp coloured by chain, side view.
PDB entry 7ugp coloured by chain, top view.
Source organisms:
Primary publication:
HIV-1 CD4-binding site germline antibody-Env structures inform vaccine design.
Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun 13 6123 (2022)
PMID: 36253376
Related structures: EMD-26493

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226239 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (9 distinct):
Envelope glycoprotein gp160 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 443 amino acids
Theoretical weight: 49.86 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S5 (Residues: 31-503; Coverage: 52%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 128 amino acids
Theoretical weight: 14.58 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S5 (Residues: 516-661; Coverage: 15%)
Gene name: env
Sequence domains: Retroviral envelope protein
10-1074 Fab heavy chain Chains: M, N, O
Molecule details ›
Chains: M, N, O
Length: 133 amino acids
Theoretical weight: 14.79 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
BG24 mature Fab heavy chain Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 120 amino acids
Theoretical weight: 13.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
10-1074 Fab light chain Chains: P, Q, R
Molecule details ›
Chains: P, Q, R
Length: 107 amino acids
Theoretical weight: 11.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
BG24 mature Fab light chain Chains: J, K, L
Molecule details ›
Chains: J, K, L
Length: 105 amino acids
Theoretical weight: 11.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Ligand NAG 6 x NAG
Ligand BMA 3 x BMA
Ligand MAN 9 x MAN
Carbohydrate polymer : NEW Components: NAG
Ligand NAG 6 x NAG
Carbohydrate polymer : NEW Components: NAG, BMA
Ligand NAG 6 x NAG
Ligand BMA 3 x BMA
1 bound ligand:
Ligand NAG 33 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-26493
Expression system: Homo sapiens

Quick links

Citations

4 citation in other articles

CD4 binding site immunogens elicit heterologous anti-HIV-1 neutralizing antibodies in transgenic and wild-type animals.
Gristick et al. (2023)
3 more