Structure analysis

Core Mediator-PICearly (Copy A)

Electron Microscopy
3.3Å resolution
Assembly composition:
hetero 33-mer (preferred)
Entry contents: 33 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 33-mer
Accessible surface area: 376638.08 Å2
Buried surface area: 145616.9 Å2
Dissociation area: 8,562.11 Å2
Dissociation energy (ΔGdiss): -7.54 kcal/mol
Dissociation entropy (TΔSdiss): 85.19 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-137342

Macromolecules

Chain: M
Length: 345 amino acids
Theoretical weight: 38.26 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P29055 (Residues: 1-345; Coverage: 100%)
Gene names: P9513.4, SUA7, YPR086W
Pfam:
InterPro:

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Chain: P
Length: 735 amino acids
Theoretical weight: 82.32 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P41895 (Residues: 1-735; Coverage: 100%)
Gene names: G7526, SSU71, TFG1, YGR186W
Pfam: Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
InterPro:

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Chain: a
Length: 566 amino acids
Theoretical weight: 64.31 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12321 (Residues: 1-566; Coverage: 100%)
Gene names: MED1, YP9499.25, YPR063W, YPR070W
Pfam: Mediator of RNA polymerase II transcription subunit 1
InterPro: Mediator complex, subunit Med1

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Chain: d
Length: 284 amino acids
Theoretical weight: 32.24 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12343 (Residues: 1-284; Coverage: 100%)
Gene names: MED4, O3630, YOR174W
Pfam: Vitamin-D-receptor interacting Mediator subunit 4
InterPro: Mediator complex, subunit Med4

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Chain: f
Length: 295 amino acids
Theoretical weight: 32.84 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38782 (Residues: 1-295; Coverage: 100%)
Gene names: MED6, MTR32, YHR058C
Pfam: MED6 mediator sub complex component
InterPro:

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Chain: g
Length: 222 amino acids
Theoretical weight: 25.62 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q08278 (Residues: 1-222; Coverage: 100%)
Gene names: MED7, YOL135C
Pfam: MED7 protein
InterPro:

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Chain: h
Length: 223 amino acids
Theoretical weight: 25.3 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38304 (Residues: 1-223; Coverage: 100%)
Gene names: MED8, YBR1403, YBR193C
Pfam: Mediator of RNA polymerase II transcription complex subunit 8
InterPro: Mediator complex, subunit Med8, fungi/metazoa

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Chain: i
Length: 149 amino acids
Theoretical weight: 17.4 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33308 (Residues: 1-149; Coverage: 100%)
Gene names: CSE2, MED9, N2046, YNR010W
Pfam: RNA polymerase II transcription mediator complex subunit 9
InterPro: Mediator of RNA polymerase II transcription subunit 9

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Chain: j
Length: 157 amino acids
Theoretical weight: 17.93 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06213 (Residues: 1-157; Coverage: 100%)
Gene names: MED10, NUT2, P9325.2, YPR168W
Pfam: Transcription factor subunit Med10 of Mediator complex
InterPro: Mediator complex, subunit Med10

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Chain: k
Length: 115 amino acids
Theoretical weight: 13.32 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q99278 (Residues: 1-115; Coverage: 100%)
Gene names: MED11, YM9718.11C, YMR112C
Pfam: Mediator complex protein
InterPro: Mediator complex, subunit Med11

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Chain: n
Length: 1082 amino acids
Theoretical weight: 123.5 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P19263 (Residues: 1-1082; Coverage: 100%)
Gene names: MED14, RGR1, YLR071C
Pfam: Mediator complex subunit MED14
InterPro: Mediator complex, subunit Med14

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Chain: q
Length: 687 amino acids
Theoretical weight: 78.58 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32569 (Residues: 1-687; Coverage: 100%)
Gene names: MED17, SRB4, YER022W
Pfam: Subunit 17 of Mediator complex
InterPro: Mediator complex, subunit Med17

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Chain: r
Length: 307 amino acids
Theoretical weight: 34.32 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32585 (Residues: 1-307; Coverage: 100%)
Gene names: MED18, SRB5, YGR104C
Pfam: Med18 protein
InterPro: Mediator complex, subunit Med18

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Chain: s
Length: 220 amino acids
Theoretical weight: 24.89 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25046 (Residues: 1-220; Coverage: 100%)
Gene names: MED19, NUT3, ROX3, SSN7, YBL0837, YBL093C
Pfam: Rox3 mediator complex subunit
InterPro: Mediator complex, subunit Med19, fungi

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Chain: t
Length: 210 amino acids
Theoretical weight: 22.92 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P34162 (Residues: 1-210; Coverage: 100%)
Gene names: HRS2, MED20, SRB2, YHR041C
Pfam: TATA-binding related factor (TRF) of subunit 20 of Mediator complex
InterPro:

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Chain: u
Length: 140 amino acids
Theoretical weight: 16.09 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P47822 (Residues: 1-140; Coverage: 100%)
Gene names: D9740.17, MED21, SRB7, YDR308C
Pfam: Subunit 21 of Mediator complex
InterPro:

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Chain: v
Length: 120 amino acids
Theoretical weight: 13.77 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32570 (Residues: 1-120; Coverage: 99%)
Gene names: MED22, SRB6, YBR1721, YBR253W
Pfam: Surfeit locus protein 5 subunit 22 of Mediator complex
InterPro:

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Chain: w
Length: 127 amino acids
Theoretical weight: 14.75 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38633 (Residues: 1-127; Coverage: 100%)
Gene names: G2864, MED31, SOH1, YGL127C
Pfam: SOH1
InterPro:

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Chain: z
Length: 25 amino acids
Theoretical weight: 2.61 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P04050 (Residues: 1666-1690; Coverage: 1%)
Gene names: D2150, RPB1, RPB220, RPO21, SUA8, YDL140C
Pfam: RNA polymerase Rpb1 C-terminal repeat
InterPro: RNA polymerase II, heptapeptide repeat, eukaryotic

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Chain: D
Length: 221 amino acids
Theoretical weight: 25.45 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P20433 (Residues: 1-221; Coverage: 100%)
Gene names: J0654, RPB4, YJL140W
Pfam: RNA polymerase Rpb4
InterPro:

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Chain: H
Length: 146 amino acids
Theoretical weight: 16.53 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P20436 (Residues: 1-146; Coverage: 100%)
Gene names: RPB8, YOR224C, YOR50-14
Pfam: RNA polymerase Rpb8
InterPro:

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Chain: J
Length: 70 amino acids
Theoretical weight: 8.29 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P22139 (Residues: 1-70; Coverage: 100%)
Gene names: RPB10, YOR210W
Pfam: RNA polymerases N / 8 kDa subunit
InterPro:

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