› 7uil

Electron Microscopy
4.3Å resolution

Mediator-PIC Early (Tail A/B Dimer)

Released:
DOI: 10.2210/pdb7uil/pdb
PDB entry 7uil coloured by chain, front view.
PDB entry 7uil coloured by chain, side view.
PDB entry 7uil coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis of a transcription pre-initiation complex on a divergent promoter.
Gorbea Colón JJ, Palao L, Chen SF, Kim HJ, Snyder L, Chang YW, Tsai KL, Murakami K
Mol Cell (2023)
PMID: 36731470
Related structures: EMD-26548

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148549 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Mediator of RNA polymerase II transcription subunit 2 Chains: 1, b
Molecule details ›
Chains: 1, b
Length: 431 amino acids
Theoretical weight: 47.75 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12124 (Residues: 1-431; Coverage: 100%)
Gene names: D2930, MED2, YDL005C
Sequence domains: Mediator complex subunit 2
Mediator of RNA polymerase II transcription subunit 3 Chains: 2, c
Molecule details ›
Chains: 2, c
Length: 397 amino acids
Theoretical weight: 43.12 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40356 (Residues: 1-397; Coverage: 100%)
Gene names: HRS1, MED3, PGD1, YGL025C
Sequence domains: Mediator complex subunit 3 fungal
Mediator of RNA polymerase II transcription subunit 5 Chains: 3, e
Molecule details ›
Chains: 3, e
Length: 1132 amino acids
Theoretical weight: 128.92 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53114 (Residues: 1-1132; Coverage: 100%)
Gene names: G1876, MED5, NUT1, YGL151W
Sequence domains: Mediator complex subunit Med5
Mediator of RNA polymerase II transcription subunit 14 Chains: 4, n
Molecule details ›
Chains: 4, n
Length: 1082 amino acids
Theoretical weight: 123.5 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P19263 (Residues: 1-1082; Coverage: 100%)
Gene names: MED14, RGR1, YLR071C
Sequence domains: Mediator complex subunit MED14
Mediator of RNA polymerase II transcription subunit 15 Chains: 5, o
Molecule details ›
Chains: 5, o
Length: 1081 amino acids
Theoretical weight: 120.43 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P19659 (Residues: 1-1081; Coverage: 100%)
Gene names: ABE1, GAL11, MED15, RAR3, SDS4, SPT13, YOL051W
Sequence domains:
Mediator of RNA polymerase II transcription subunit 16 Chains: 6, p
Molecule details ›
Chains: 6, p
Length: 974 amino acids
Theoretical weight: 111.42 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32259 (Residues: 1-974; Coverage: 100%)
Gene names: BEL2, GAL22, MED16, N1135, RYE1, SDI3, SIN4, SSF5, SSN4, TSF3, YNL236W
Sequence domains:
Mediator of RNA polymerase II transcription subunit 1 Chain: a
Molecule details ›
Chain: a
Length: 566 amino acids
Theoretical weight: 64.31 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12321 (Residues: 1-566; Coverage: 100%)
Gene names: MED1, YP9499.25, YPR063W, YPR070W
Sequence domains: Mediator of RNA polymerase II transcription subunit 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-26548

Quick links

Citations

3 review citations

Regulation of the RNA polymerase II pre-initiation complex by its associated coactivators.
Malik et al. (2023)
2 more

1 mention without citation

Structural basis of a transcription pre-initiation complex on a divergent promoter.
Gorbea Colón et al. (2023)