› 7ul2

Electron Microscopy
2.4Å resolution

CryoEM Structure of Inactive NTSR1 Bound to SR48692 and Nb6

Released:
DOI: 10.2210/pdb7ul2/pdb
PDB entry 7ul2 coloured by chain, front view.
PDB entry 7ul2 coloured by chain, side view.
PDB entry 7ul2 coloured by chain, top view.
Source organisms:
Primary publication:
Structure determination of inactive-state GPCRs with a universal nanobody.
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022)
PMID: 36396979
Related structures: EMD-26589

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225587 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kappa-type opioid receptor; Neurotensin receptor type 1 Chain: R
Molecule details ›
Chain: R
Length: 419 amino acids
Theoretical weight: 47.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30989 (Residues: 20-255, 307-418; Coverage: 83%)
  • Canonical: P41145 (Residues: 246-277; Coverage: 8%)
Gene names: NTRR, NTSR1, OPRK, OPRK1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Nanobody 6 Chain: D
Molecule details ›
Chain: D
Length: 133 amino acids
Theoretical weight: 14.73 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand Q6Q 1 x Q6Q
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.4Å
Relevant EMDB volumes: EMD-26589
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

7 review citations

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
6 more

2 mentions without citation

Putting on molecular weight: Enabling cryo-EM structure determination of sub-100-kDa proteins.
Wentinck et al. (2022)
1 more