› 7uur

Electron Microscopy
1.67Å resolution

The 1.67 Angstrom CryoEM structure of the [NiFe]-hydrogenase Huc from Mycobacterium smegmatis - catalytic dimer (Huc2S2L)

Released:
DOI: 10.2210/pdb7uur/pdb
PDB entry 7uur coloured by chain, front view.
PDB entry 7uur coloured by chain, side view.
PDB entry 7uur coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structural basis for bacterial energy extraction from atmospheric hydrogen.
Grinter R, Kropp A, Venugopal H, Senger M, Badley J, Cabotaje PR, Jia R, Duan Z, Huang P, Stripp ST, Barlow CK, Belousoff M, Shafaat HS, Cook GM, Schittenhelm RB, Vincent KA, Khalid S, Berggren G, Greening C
OpenAccess logo Nature (2023)
PMID: 36890228
Related structures: EMD-26801

Function and Biology Details

Reaction catalysed: 
H(2) + A = AH(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222101 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hydrogenase-2, large subunit Chains: C, F
Molecule details ›
Chains: C, F
Length: 513 amino acids
Theoretical weight: 57.22 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QUM7 (Residues: 4-516; Coverage: 96%)
Gene names: MSMEG_2263, hybC
Sequence domains: Nickel-dependent hydrogenase
Hydrogenase-2, small subunit Chains: D, G
Molecule details ›
Chains: D, G
Length: 322 amino acids
Theoretical weight: 35.07 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QUM6 (Residues: 2-323; Coverage: 100%)
Gene names: MSMEG_2262, hybA
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand 3NI 2 x 3NI
Ligand FCO 2 x FCO
Ligand OH 2 x OH
Ligand MG 2 x MG
Ligand VK3 2 x VK3
Ligand F3S 6 x F3S
1 modified residue:
Ligand DHI 2 x DHI

Experiments and Validation Details

Entry percentile scores
Resolution: 1.67Å
Relevant EMDB volumes: EMD-26801
Expression system: Mycolicibacterium smegmatis MC2 155

Quick links

Citations

2 review citations

Clearing the air: unraveling past and guiding future research in atmospheric chemosynthesis.
Ray et al. (2023)
1 more

1 mention without citation

Developing high-affinity, oxygen-insensitive [NiFe]-hydrogenases as biocatalysts for energy conversion.
Greening et al. (2023)