› 7vgx

Electron Microscopy
3.2Å resolution

Neuropeptide Y Y1 Receptor (NPY1R) in Complex with G Protein and its endogeneous Peptide-Agonist Neuropeptide Y (NPY)

Released:
DOI: 10.2210/pdb7vgx/pdb
PDB entry 7vgx coloured by chain, front view.
PDB entry 7vgx coloured by chain, side view.
PDB entry 7vgx coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of neuropeptide Y signaling through Y1 receptor
Park C, Kim J, Ko SB, Choi YK, Jeong H, Woo H, Kang H, Bang I, Kim SA, Yoon TY, Seok C, Im W, Choi HJ
Nat Commun 13 853- (2022)
Related structures: EMD-31979

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-208392 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Neuropeptide Y receptor type 1 Chain: R
Molecule details ›
Chain: R
Length: 402 amino acids
Theoretical weight: 46.49 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P25929 (Residues: 1-384; Coverage: 100%)
Gene names: NPY1R, NPYR, NPYY1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Neuropeptide Y Chain: L
Molecule details ›
Chain: L
Length: 36 amino acids
Theoretical weight: 4.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01303 (Residues: 29-64; Coverage: 52%)
Gene name: NPY
Sequence domains: Pancreatic hormone peptide
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 40.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63096 (Residues: 2-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
single-chain antibody Fv fragment (scFv16) Chain: S
Molecule details ›
Chain: S
Length: 259 amino acids
Theoretical weight: 27.78 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-31979
Expression systems:
  • Spodoptera frugiperda
  • Not provided
  • Escherichia coli
  • Trichoplusia ni

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