Assemblies
Multimeric state:
hetero undecamer
Accessible surface area:
167025.02 Å2
Buried surface area:
65391.31 Å2
Dissociation area:
1,521.15
Å2
Dissociation energy (ΔGdiss):
8.71
kcal/mol
Dissociation entropy (TΔSdiss):
14.13
kcal/mol
Symmetry number:
1
PDBe Complex ID:
PDB-CPX-105604
Macromolecules
Chains: A, B
Length: 340 amino acids
Theoretical weight: 36.73 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
Length: 340 amino acids
Theoretical weight: 36.73 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
A0A6G2M9E1 (Residues: 1-340; Coverage: 100%)
Pfam:
- RNA polymerase Rpb3/Rpb11 dimerisation domain
- RNA polymerase Rpb3/RpoA insert domain
- Bacterial RNA polymerase, alpha chain C terminal domain
Chain: C
Length: 1161 amino acids
Theoretical weight: 128.64 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
Length: 1161 amino acids
Theoretical weight: 128.64 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q9L0L0 (Residues: 1-1161; Coverage: 100%)
Pfam:
- RNA polymerase beta subunit
- RNA polymerase Rpb2, domain 2
- RNA polymerase Rpb2, domain 3
- RNA polymerase beta subunit external 1 domain
- RNA polymerase Rpb2, domain 6
- RNA polymerase Rpb2, domain 7
- DNA-directed RNA polymerase beta subunit, bacterial-type
- DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily
- RNA polymerase, beta subunit, protrusion
- DNA-directed RNA polymerase, subunit 2
- RNA polymerase Rpb2, domain 2
- RNA polymerase Rpb2, domain 2 superfamily
- RNA polymerase Rpb2, domain 3
- DNA-directed RNA polymerase, beta subunit, external 1 domain
- DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily
- DNA-directed RNA polymerase, subunit 2, hybrid-binding domain
- RNA polymerase Rpb2, OB-fold
- RNA polymerase, beta subunit, conserved site
- RNA polymerase Rpb2, domain 7
Chain: D
Length: 1307 amino acids
Theoretical weight: 145.91 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
Length: 1307 amino acids
Theoretical weight: 145.91 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q8CJT1 (Residues: 1-1299; Coverage: 100%)
Pfam:
- RNA polymerase Rpb1, domain 1
- RNA polymerase Rpb1, domain 2
- RNA polymerase Rpb1, domain 3
- RNA polymerase Rpb1, domain 4
- RNA polymerase Rpb1, domain 5
- RNA polymerase Rpb1, clamp domain superfamily
- DNA-directed RNA polymerase, subunit beta-prime, bacterial type
- RNA polymerase Rpb1, domain 1
- DNA-directed RNA polymerase, subunit beta-prime
- RNA polymerase, N-terminal
- RNA polymerase, alpha subunit
- RNA polymerase Rpb1, domain 3
- RNA polymerase Rpb1, domain 3 superfamily
- RNA polymerase Rpb1, funnel domain superfamily
- RNA polymerase Rpb1, domain 4
- RNA polymerase Rpb1, domain 5
Chain: E
Length: 90 amino acids
Theoretical weight: 9.72 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam: RNA polymerase Rpb6
InterPro:
Length: 90 amino acids
Theoretical weight: 9.72 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q9KXS1 (Residues: 1-90; Coverage: 100%)
Pfam: RNA polymerase Rpb6
InterPro:
Chain: F
Length: 531 amino acids
Theoretical weight: 58.19 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
InterPro:
Length: 531 amino acids
Theoretical weight: 58.19 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P18183 (Residues: 1-511; Coverage: 100%)
Pfam:
InterPro:
- Winged helix-like DNA-binding domain superfamily
- RNA polymerase sigma factor RpoD
- RNA polymerase sigma factor, region 2
- RNA polymerase sigma-70 region 1.2
- RNA polymerase sigma factor RpoD, C-terminal
- RNA polymerase sigma-70-like domain
- RNA polymerase sigma-70 region 2
- RNA polymerase sigma-70
- RNA polymerase sigma factor, region 3/4-like
- RNA polymerase sigma-70 region 3
- RNA polymerase sigma-70 region 4
Chains: M, N
Length: 159 amino acids
Theoretical weight: 16.9 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam: Ferric uptake regulator family
InterPro:
Length: 159 amino acids
Theoretical weight: 16.9 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q9L2H5 (Residues: 1-139; Coverage: 100%)
Pfam: Ferric uptake regulator family
InterPro:
Name:
DNA (84-MER)
Representative chains: O
Length: 84 nucleotides
Theoretical weight: 25.81 KDa
Representative chains: O
Length: 84 nucleotides
Theoretical weight: 25.81 KDa
Name:
DNA (84-MER)
Representative chains: P
Length: 84 nucleotides
Theoretical weight: 26.01 KDa
Representative chains: P
Length: 84 nucleotides
Theoretical weight: 26.01 KDa
