Structure analysis

Active state CI from Q10 dataset, Subclass 2

Electron Microscopy
3.2Å resolution
Source organism: Sus scrofa
Assembly composition:
hetero 45-mer (preferred)
Entry contents: 44 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 45-mer
PDBe Complex ID: PDB-CPX-236692

Macromolecules

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Chain: B
Length: 176 amino acids
Theoretical weight: 20.21 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287BDC0 (Residues: 37-212; Coverage: 83%)
Gene name: NDUFS8
Pfam: 4Fe-4S dicluster domain
InterPro:

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Chain: C
Length: 156 amino acids
Theoretical weight: 17.87 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1TL60 (Residues: 41-196; Coverage: 80%)
Gene name: NDUFS7
Pfam: NADH ubiquinone oxidoreductase, 20 Kd subunit
InterPro:

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Chain: E
Length: 115 amino acids
Theoretical weight: 13.81 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SJP6 (Residues: 40-154; Coverage: 75%)
Gene name: NDUFA6
Pfam: Complex 1 protein (LYR family)
InterPro:

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Chain: F
Length: 86 amino acids
Theoretical weight: 9.84 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1RGE3 (Residues: 14-99; Coverage: 87%)
Gene name: NDUFA2
Pfam: Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain
InterPro:

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Chains: G, X
Length: 88 amino acids
Theoretical weight: 10.13 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287AAR5 (Residues: 69-156; Coverage: 56%)
Gene name: NDUFAB1
Pfam: Phosphopantetheine attachment site
InterPro:

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Chain: H
Length: 112 amino acids
Theoretical weight: 12.95 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287BAW0 (Residues: 5-116; Coverage: 97%)
Gene name: NDUFA5
Pfam: ETC complex I subunit conserved region
InterPro: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

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Chain: I
Length: 112 amino acids
Theoretical weight: 12.52 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8W4F7N8 (Residues: 2-113; Coverage: 99%)
Gene name: NDUFA7
Pfam: NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a)
InterPro: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

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Chain: J
Length: 342 amino acids
Theoretical weight: 38.95 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287AE88 (Residues: 36-407; Coverage: 84%)
Gene name: NDUFA9
Pfam: NAD dependent epimerase/dehydratase family
InterPro:

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Chain: K
Length: 43 amino acids
Theoretical weight: 5.19 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: I3LRR4 (Residues: 68-110; Coverage: 39%)
Gene name: NDUFV3
Pfam: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
InterPro: NADH-ubiquinone oxidoreductase flavoprotein 3

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Chain: L
Length: 125 amino acids
Theoretical weight: 14.44 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: G8IFA6 (Residues: 51-175; Coverage: 71%)
Pfam: NADH dehydrogenase ubiquinone Fe-S protein 4
InterPro:

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Chain: N
Length: 144 amino acids
Theoretical weight: 17.03 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SQP4 (Residues: 2-145; Coverage: 99%)
Gene name: NDUFA12
Pfam: NADH ubiquinone oxidoreductase subunit NDUFA12
InterPro: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

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Chain: O
Length: 217 amino acids
Theoretical weight: 23.83 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287BG40 (Residues: 33-249; Coverage: 87%)
Gene name: NDUFV2
Pfam: Thioredoxin-like [2Fe-2S] ferredoxin
InterPro:

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Chain: S
Length: 70 amino acids
Theoretical weight: 8.09 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: K7GR43 (Residues: 1-70; Coverage: 100%)
Gene name: NDUFA1
Pfam: NADH-ubiquinone oxidoreductase MWFE subunit
InterPro: NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1

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Chain: T
Length: 96 amino acids
Theoretical weight: 10.57 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1S031 (Residues: 28-123; Coverage: 78%)
Gene name: NDUFS6
Pfam: Zinc-finger domain
InterPro:

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Chain: U
Length: 83 amino acids
Theoretical weight: 9.09 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VQ42 (Residues: 2-84; Coverage: 99%)
Gene name: NDUFA3
Pfam: NADH dehydrogenase 1 alpha subcomplex subunit 3
InterPro: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

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Chain: V
Length: 140 amino acids
Theoretical weight: 14.56 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: I3LGM4 (Residues: 2-141; Coverage: 99%)
Gene name: NDUFA11
Pfam: Tim17/Tim22/Tim23/Pmp24 family
InterPro: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

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Chain: W
Length: 142 amino acids
Theoretical weight: 16.71 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1NRT6 (Residues: 3-144; Coverage: 99%)
Gene name: NDUFA13
Pfam: GRIM-19 protein
InterPro: GRIM-19

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Chain: Y
Length: 67 amino acids
Theoretical weight: 8.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A5G2QW32 (Residues: 52-118; Coverage: 56%)
Gene name: NDUFB2
Pfam: NADH dehydrogenase 1 beta subcomplex subunit 2
InterPro: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type

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Chain: Z
Length: 80 amino acids
Theoretical weight: 9.04 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VD84 (Residues: 12-91; Coverage: 82%)
Gene name: NDUFB3
Pfam: NADH-ubiquinone oxidoreductase B12 subunit family
InterPro: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

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Chain: a
Length: 138 amino acids
Theoretical weight: 16.28 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SGC6 (Residues: 52-189; Coverage: 73%)
Gene name: NDUFB5
Pfam: NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit
InterPro: NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit

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Chain: b
Length: 126 amino acids
Theoretical weight: 15.33 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A480KM09 (Residues: 62-187; Coverage: 67%)
Gene name: NDUFB6
Pfam: NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit
InterPro: NADH dehydrogenase 1, beta subcomplex, subunit 6

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Chain: c
Length: 156 amino acids
Theoretical weight: 18.61 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1RWL7 (Residues: 31-186; Coverage: 84%)
Gene name: NDUFB8
Pfam: NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8)
InterPro:

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Chain: d
Length: 175 amino acids
Theoretical weight: 20.81 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: I3LDC3 (Residues: 2-201; Coverage: 87%)
Gene name: NDUFB10
Pfam: NADH-ubiquinone oxidoreductase subunit 10
InterPro:

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Chain: e
Length: 104 amino acids
Theoretical weight: 12.31 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287BMW6 (Residues: 51-164; Coverage: 71%)
Gene name: NDUFB11
Pfam: ESSS subunit of NADH:ubiquinone oxidoreductase (complex I)
InterPro: NADH:ubiquinone oxidoreductase, ESSS subunit

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Chain: f
Length: 49 amino acids
Theoretical weight: 5.88 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A1XQS6 (Residues: 28-76; Coverage: 65%)
Gene name: NDUFC1
Pfam: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
InterPro: NADH-ubiquinone oxidoreductase 1 subunit C1

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Chain: g
Length: 121 amino acids
Theoretical weight: 14.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A480JRW3 (Residues: 2-122; Coverage: 99%)
Gene name: NDUFC2
Pfam: NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2)
InterPro: NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2

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Chain: h
Length: 105 amino acids
Theoretical weight: 12.38 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SV23 (Residues: 2-106; Coverage: 99%)
Gene name: NDUFS5
Pfam: NADH:ubiquinone oxidoreductase, NDUFS5-15kDa
InterPro: NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5

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Chain: i
Length: 347 amino acids
Theoretical weight: 39.08 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: O79875 (Residues: 1-347; Coverage: 100%)
Gene names: MT-ND2, MTND2, NADH2, ND2
Pfam:
InterPro:

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Chain: j
Length: 115 amino acids
Theoretical weight: 13 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: O79880 (Residues: 1-115; Coverage: 100%)
Gene names: MT-ND3, MTND3, NADH3, ND3
Pfam: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
InterPro:

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Chain: k
Length: 98 amino acids
Theoretical weight: 10.83 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P56632 (Residues: 1-98; Coverage: 100%)
Gene names: MT-ND4L, MTND4L, NADH4L, ND4L
Pfam: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
InterPro:

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Chain: m
Length: 175 amino acids
Theoretical weight: 19.02 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: O79882 (Residues: 1-175; Coverage: 100%)
Gene names: MT-ND6, MTND6, NADH6, ND6
Pfam: NADH-ubiquinone/plastoquinone oxidoreductase chain 6
InterPro:

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Chain: n
Length: 56 amino acids
Theoretical weight: 6.78 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D2CGH8 (Residues: 3-58; Coverage: 97%)
Gene name: NDUFB1
Pfam: MNLL subunit
InterPro: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1

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Chain: o
Length: 128 amino acids
Theoretical weight: 14.99 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1T6M0 (Residues: 2-129; Coverage: 99%)
Gene name: NDUFB4
Pfam: NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4)
InterPro: NADH:ubiquinone oxidoreductase, subunit NDUFB4

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Chain: p
Length: 178 amino acids
Theoretical weight: 21.73 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: K7GSE5 (Residues: 44-221; Coverage: 81%)
Gene name: NDUFB9
Pfam: Complex 1 protein (LYR family)
InterPro:

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Chain: s
Length: 318 amino acids
Theoretical weight: 35.67 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: O79874 (Residues: 1-318; Coverage: 100%)
Gene names: MT-ND1, MTND1, NADH1, ND1
Pfam: NADH dehydrogenase
InterPro:

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Chain: u
Length: 171 amino acids
Theoretical weight: 19.93 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SLR1 (Residues: 79-249; Coverage: 69%)
Gene name: NDUFA8
Pfam: CHCH domain
InterPro:

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Chain: v
Length: 124 amino acids
Theoretical weight: 15.18 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SCH1 (Residues: 2-125; Coverage: 91%)
Gene name: NDUFB7
Pfam: NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7)
InterPro: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7

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Chain: w
Length: 320 amino acids
Theoretical weight: 36.79 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SIS9 (Residues: 38-357; Coverage: 90%)
Gene name: NDUFA10
Pfam: Deoxynucleoside kinase
InterPro:

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