› 7w77

Electron Microscopy
3.3Å resolution

cryo-EM structure of human NaV1.3/beta1/beta2-bulleyaconitineA

Released:
DOI: 10.2210/pdb7w77/pdb
PDB entry 7w77 coloured by chain, front view.
PDB entry 7w77 coloured by chain, side view.
PDB entry 7w77 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for modulation of human NaV1.3 by clinical drug and selective antagonist.
Li X, Xu F, Xu H, Zhang S, Gao Y, Zhang H, Dong Y, Zheng Y, Yang B, Sun J, Zhang XC, Zhao Y, Jiang D
Nat Commun 13 1286 (2022)
PMID: 35277491
Related structures: EMD-32341

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130220 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Sodium channel subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 218 amino acids
Theoretical weight: 24.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q07699 (Residues: 1-218; Coverage: 100%)
Gene name: SCN1B
Sequence domains: Immunoglobulin V-set domain
Sodium channel subunit beta-2 Chain: C
Molecule details ›
Chain: C
Length: 215 amino acids
Theoretical weight: 24.36 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O60939 (Residues: 1-215; Coverage: 100%)
Gene names: SCN2B, UNQ326/PRO386
Sequence domains: Immunoglobulin V-set domain
Sodium channel protein type 3 subunit alpha Chain: D
Molecule details ›
Chain: D
Length: 1951 amino acids
Theoretical weight: 221.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NY46 (Residues: 1-1951; Coverage: 98%)
Gene names: KIAA1356, NAC3, SCN3A
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 5 x NAG
Ligand 9Z9 3 x 9Z9
Ligand 6OU 16 x 6OU
Ligand 966 1 x 966
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-32341
Expression system: Homo sapiens

Quick links

Citations

16 citation in other articles

Design, synthesis, and mechanism of action of novel μ-conotoxin KIIIA analogues for inhibition of the voltage-gated sodium channel Nav1.7.
Zhao et al. (2023)
15 more

2 mentions without citation

A structural atlas of druggable sites on Nav channels.
Li et al. (2024)
1 more