› 7wht

Electron Microscopy
3.5Å resolution

Cryo-EM Structure of Leishmanial GDP-mannose pyrophosphorylase in complex with GDP-Mannose

Released:
DOI: 10.2210/pdb7wht/pdb
PDB entry 7wht coloured by chain, front view.
PDB entry 7wht coloured by chain, side view.
PDB entry 7wht coloured by chain, top view.
Source organism: Leishmania donovani
Primary publication:
Structural insights into selective inhibition of leishmanial GDP-mannose pyrophosphorylase.
Li H, Ji T, Sun Q, Chen Y, Xu W, Huang C
OpenAccess logo Cell Discov 8 83 (2022)
PMID: 36038534
Related structures: EMD-32511

Function and Biology Details

Reaction catalysed: 
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123161 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mannose-1-phosphate guanylyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 379 amino acids
Theoretical weight: 41.78 KDa
Source organism: Leishmania donovani
Expression system: Escherichia coli
UniProt:
  • Canonical: E9BG32 (Residues: 1-379; Coverage: 100%)
Gene names: CGC21_17635, LDBPK_230120
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GDD 6 x GDD
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-32511
Expression system: Escherichia coli

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