› 7wn1

Electron Microscopy
3.11Å resolution

Structure of PfNT1(Y190A) in complex with nanobody 48 and inosine

Released:
DOI: 10.2210/pdb7wn1/pdb
PDB entry 7wn1 coloured by chain, front view.
PDB entry 7wn1 coloured by chain, side view.
PDB entry 7wn1 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of the substrate recognition and inhibition mechanism of Plasmodium falciparum nucleoside transporter PfENT1.
Wang C, Yu L, Zhang J, Zhou Y, Sun B, Xiao Q, Zhang M, Liu H, Li J, Li J, Luo Y, Xu J, Lian Z, Lin J, Wang X, Zhang P, Guo L, Ren R, Deng D
OpenAccess logo Nat Commun 14 1727 (2023)
PMID: 36977719
Related structures: EMD-32619

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226976 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleoside transporter 1 Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 47.58 KDa
Source organism: Plasmodium falciparum
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8IDM6 (Residues: 1-422; Coverage: 100%)
Gene names: NT1, PF3D7_1347200
nanobody48 Chain: C
Molecule details ›
Chain: C
Length: 122 amino acids
Theoretical weight: 13.2 KDa
Source organism: Vicugna pacos
Expression system: Bacillus subtilis

Ligands and Environments

1 bound ligand:
Ligand NOS 1 x NOS
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.11Å
Relevant EMDB volumes: EMD-32619
Expression systems:
  • Spodoptera frugiperda
  • Bacillus subtilis

Quick links

Citations

1 review citation

Critical interdependencies between Plasmodium nutrient flux and drugs.
Henshall et al. (2023)
2 other articles