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Structure of PfNT1(Y190A) in complex with nanobody 48 and inosine

Electron Microscopy
3.11Å resolution
PDB entry 7wn1 coloured by chain, front view.
PDB entry 7wn1 coloured by chain, side view.
PDB entry 7wn1 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Nucleoside transporter 1 and IG-heavy chain
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Multimeric state: hetero dimer
Accessible surface area: 20973.95 Å2
Buried surface area: 3166.84 Å2
Dissociation area: 1,547.26 Å2
Dissociation energy (ΔGdiss): 3.74 kcal/mol
Dissociation entropy (TΔSdiss): 12.26 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-280282

Macromolecules

Nucleoside transporter 1
Chain: A
Length: 422 amino acids
Theoretical weight: 47.58 KDa
Source organism: Plasmodium falciparum
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8IDM6 (Residues: 1-422; Coverage: 100%)
Gene names: NT1, PF3D7_1347200
InterPro:
Equilibrative nucleoside/nucleobase transporter in PDB entry 7wn1, assembly 1, front view.
Equilibrative nucleoside/nucleobase transporter in PDB entry 7wn1, assembly 1, side view.
Equilibrative nucleoside/nucleobase transporter in PDB entry 7wn1, assembly 1, top view.
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nanobody48
Chain: C
Length: 122 amino acids
Theoretical weight: 13.2 KDa
Source organism: Vicugna pacos
Expression system: Bacillus subtilis
InterPro:
nanobody48 in PDB entry 7wn1, assembly 1, front view.
nanobody48 in PDB entry 7wn1, assembly 1, side view.
nanobody48 in PDB entry 7wn1, assembly 1, top view.
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