› 7wsg

Electron Microscopy
3.03Å resolution

Cryo-EM structure of SARS-CoV spike receptor-binding domain in complex with sea lion ACE2

Released:
DOI: 10.2210/pdb7wsg/pdb
PDB entry 7wsg coloured by chain, front view.
PDB entry 7wsg coloured by chain, side view.
PDB entry 7wsg coloured by chain, top view.
Source organisms:
Primary publication:
Cross-species recognition and molecular basis of SARS-CoV-2 and SARS-CoV binding to ACE2s of marine animals.
Li S, Yang R, Zhang D, Han P, Xu Z, Chen Q, Zhao R, Zhao X, Qu X, Zheng A, Wang L, Li L, Hu Y, Zhang R, Su C, Niu S, Zhang Y, Qi J, Liu K, Wang Q, Gao GF
OpenAccess logo Natl Sci Rev 9 nwac122 (2022)
PMID: 36187898
Related structures: EMD-32757

Function and Biology Details

Reaction catalysed: 
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105660 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Angiotensin-converting enzyme Chain: A
Molecule details ›
Chain: A
Length: 806 amino acids
Theoretical weight: 93.01 KDa
Source organism: Mammalia
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6J2EID0 (Residues: 1-806; Coverage: 100%)
Gene name: ACE2
Sequence domains:
Spike protein S1 Chain: B
Molecule details ›
Chain: B
Length: 190 amino acids
Theoretical weight: 21.62 KDa
Source organism: Severe acute respiratory syndrome coronavirus
Expression system: Homo sapiens
UniProt:
  • Canonical: P59594 (Residues: 321-502; Coverage: 15%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.03Å
Relevant EMDB volumes: EMD-32757
Expression systems:
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

4 citation in other articles

Beluga whale and bottlenose dolphin ACE2 proteins allow cell entry mediated by spike protein from three variants of SARS-CoV-2.
Stone et al. (2023)
3 more

1 mention without citation

Cross-species recognition and molecular basis of SARS-CoV-2 and SARS-CoV binding to ACE2s of marine animals.
Li et al. (2022)