› 7wza

X-ray diffraction
1.5Å resolution

An open conformation Form 1 of switch II for RhoA

Released:
DOI: 10.2210/pdb7wza/pdb
PDB entry 7wza coloured by chain, front view.
PDB entry 7wza coloured by chain, side view.
PDB entry 7wza coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A RhoA structure with switch II flipped outward revealed the conformational dynamics of switch II region.
Jiang H, Zu S, Lu Y, Sun Z, Adeerjiang A, Guo Q, Zhang H, Dong C, Wu Q, Ding H, Du D, Wang M, Liu C, Tang Y, Liang Z, Luo C
J Struct Biol 215 107942 (2023)
PMID: 36781028

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transforming protein RhoA Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61586 (Residues: 1-181; Coverage: 94%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 31.412Å b: 67.368Å c: 83.529Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.22
Expression system: Escherichia coli

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