› 7x7a

Electron Microscopy
3.2Å resolution

Cryo-EM structure of SbCas7-11 in complex with crRNA and target RNA

Released:
DOI: 10.2210/pdb7x7a/pdb
PDB entry 7x7a coloured by chain, front view.
PDB entry 7x7a coloured by chain, side view.
PDB entry 7x7a coloured by chain, top view.
Source organism: Candidatus Scalindua brodae
Primary publication:
Structure and function of a bacterial type III-E CRISPR-Cas7-11 complex.
Yu G, Wang X, Zhang Y, An Q, Wen Y, Li X, Yin H, Deng Z, Zhang H
Nat Microbiol (2022)
PMID: 36302881
Related structures: EMD-33040

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100874 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RAMP superfamily protein Chain: A
Molecule details ›
Chain: A
Length: 1722 amino acids
Theoretical weight: 197.82 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B0EGF3 (Residues: 1-1717; Coverage: 100%)
Gene name: SCABRO_02597
Sequence domains: RAMP superfamily
RNA (33-MER) Chain: C
Molecule details ›
Chain: C
Length: 33 nucleotides
Theoretical weight: 10.4 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-33040
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future.
Zhang et al. (2023)
4 more