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Cryo-EM structure of SbCas7-11 in complex with crRNA and target RNA

Electron Microscopy
3.2Å resolution
PDB entry 7x7a coloured by chain, front view.
PDB entry 7x7a coloured by chain, side view.
PDB entry 7x7a coloured by chain, top view.
Source organism: Candidatus Scalindua brodae
Assembly composition:
hetero dimer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: RAMP superfamily protein and RNA
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Multimeric state: hetero dimer
Accessible surface area: 50823.14 Å2
Buried surface area: 11682.21 Å2
Dissociation area: 5,841.11 Å2
Dissociation energy (ΔGdiss): 118.32 kcal/mol
Dissociation entropy (TΔSdiss): 14.47 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-100874

Macromolecules

RAMP superfamily protein
Chain: A
Length: 1722 amino acids
Theoretical weight: 197.82 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B0EGF3 (Residues: 1-1717; Coverage: 100%)
Gene name: SCABRO_02597
Pfam: RAMP superfamily
InterPro: CRISPR type III-associated protein
RAMP superfamily protein in PDB entry 7x7a, assembly 1, front view.
RAMP superfamily protein in PDB entry 7x7a, assembly 1, side view.
RAMP superfamily protein in PDB entry 7x7a, assembly 1, top view.
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RNA chain C
Name: RNA (33-MER)
Representative chains: C
Source organism: Candidatus Scalindua brodae [237368]
Expression system: Escherichia coli BL21(DE3) [469008]
Length: 33 nucleotides
Theoretical weight: 10.4 KDa
RNA (33-MER) in PDB entry 7x7a, assembly 1, front view.
RNA (33-MER) in PDB entry 7x7a, assembly 1, side view.
RNA (33-MER) in PDB entry 7x7a, assembly 1, top view.
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