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Cryo-EM structure of a bacterial protein complex

Electron Microscopy
2.8Å resolution
PDB entry 7x8a coloured by chain, front view.
PDB entry 7x8a coloured by chain, side view.
PDB entry 7x8a coloured by chain, top view.
Source organism: Candidatus Scalindua brodae
Assembly composition:
hetero trimer (preferred)
Entry contents: 2 distinct polypeptide molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CHAT domain-containing protein and RAMP superfamily protein and RNA
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Multimeric state: hetero trimer
Accessible surface area: 76008.24 Å2
Buried surface area: 15119.71 Å2
Dissociation area: 1,657.46 Å2
Dissociation energy (ΔGdiss): 5.95 kcal/mol
Dissociation entropy (TΔSdiss): 15.96 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-220990

Macromolecules

CHAT domain-containing protein
Chain: D
Length: 716 amino acids
Theoretical weight: 82.55 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B0EKL4 (Residues: 1-716; Coverage: 100%)
Gene name: SCABRO_02601
Pfam: CHAT domain
InterPro: CHAT domain
CHAT domain-containing protein in PDB entry 7x8a, assembly 1, front view.
CHAT domain-containing protein in PDB entry 7x8a, assembly 1, side view.
CHAT domain-containing protein in PDB entry 7x8a, assembly 1, top view.
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RAMP superfamily protein
Chain: A
Length: 1722 amino acids
Theoretical weight: 197.82 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B0EGF3 (Residues: 1-1717; Coverage: 100%)
Gene name: SCABRO_02597
Pfam: RAMP superfamily
InterPro: CRISPR type III-associated protein
RAMP superfamily protein in PDB entry 7x8a, assembly 1, front view.
RAMP superfamily protein in PDB entry 7x8a, assembly 1, side view.
RAMP superfamily protein in PDB entry 7x8a, assembly 1, top view.
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RNA chain C
Name: RNA (33-MER)
Representative chains: C
Length: 33 nucleotides
Theoretical weight: 10.4 KDa
RNA (33-MER) in PDB entry 7x8a, assembly 1, front view.
RNA (33-MER) in PDB entry 7x8a, assembly 1, side view.
RNA (33-MER) in PDB entry 7x8a, assembly 1, top view.
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