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Structure of the SecA/SecYE/proOmpA(4Y)-sfGFP complex with ADP.BeF3-.

Electron Microscopy
3.35Å resolution
PDB entry 7xha coloured by chain, front view.
PDB entry 7xha coloured by chain, side view.
PDB entry 7xha coloured by chain, top view.
Source organisms:
Assembly composition:
hetero tetramer (preferred)
Entry contents: 4 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tetramer
Accessible surface area: 58624.24 Å2
Buried surface area: 14434.94 Å2
Dissociation area: 1,587.7 Å2
Dissociation energy (ΔGdiss): 24.85 kcal/mol
Dissociation entropy (TΔSdiss): 12.28 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-107330

Macromolecules

Protein translocase subunit SecA
Chain: A
Length: 778 amino acids
Theoretical weight: 88.67 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P28366 (Residues: 1-778; Coverage: 93%)
Gene names: BSU35300, div+, secA
Pfam:
InterPro:
Protein translocase subunit SecA in PDB entry 7xha, assembly 1, front view.
Protein translocase subunit SecA in PDB entry 7xha, assembly 1, side view.
Protein translocase subunit SecA in PDB entry 7xha, assembly 1, top view.
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Protein translocase subunit SecY
Chain: Y
Length: 430 amino acids
Theoretical weight: 47.63 KDa
Source organism: Geobacillus thermodenitrificans NG80-2
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A4IJK8 (Residues: 1-430; Coverage: 100%)
Gene names: GTNG_0125, secY
Pfam: SecY
InterPro:
Protein translocase subunit SecY in PDB entry 7xha, assembly 1, front view.
Protein translocase subunit SecY in PDB entry 7xha, assembly 1, side view.
Protein translocase subunit SecY in PDB entry 7xha, assembly 1, top view.
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Protein translocase subunit SecE
Chain: E
Length: 60 amino acids
Theoretical weight: 7.03 KDa
Source organism: Geobacillus thermodenitrificans NG80-2
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A4IJH4 (Residues: 1-60; Coverage: 100%)
Gene names: GTNG_0091, secE
Pfam: SecE/Sec61-gamma subunits of protein translocation complex
InterPro:
Protein translocase subunit SecE in PDB entry 7xha, assembly 1, front view.
Protein translocase subunit SecE in PDB entry 7xha, assembly 1, side view.
Protein translocase subunit SecE in PDB entry 7xha, assembly 1, top view.
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Translocating polypeptide
Chain: B
Length: 303 amino acids
Theoretical weight: 33.18 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
InterPro:
Translocating polypeptide in PDB entry 7xha, assembly 1, front view.
Translocating polypeptide in PDB entry 7xha, assembly 1, side view.
Translocating polypeptide in PDB entry 7xha, assembly 1, top view.
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