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Pseudomonas aeruginosa SutA wedges RNAP lobe domain open to facilitate promoter DNA unwinding.

<div class='caption-title'>The requirement of SutA domains in RNAP interaction and transcription activation.</div>
<div class='caption-title'>The cryo-EM structure of Pae RNAP-σS holoenzyme.</div>
<div class='caption-title'>The cryo-EM structure of Pae SutA-RNAP-σS complex.</div>
He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y
OpenAccess logo Nat Commun 13 4204 (2022)
Related entries: 7f0r, 7vf9, 7xl3

Cited: 6 times
EuropePMC logo PMID: 35859063

Abstract

Pseudomonas aeruginosa (Pae) SutA adapts bacteria to hypoxia and nutrition-limited environment during chronic infection by increasing transcription activity of an RNA polymerase (RNAP) holoenzyme comprising the stress-responsive σ factor σS (RNAP-σS). SutA shows no homology to previously characterized RNAP-binding proteins. The structure and mode of action of SutA remain unclear. Here we determined cryo-EM structures of Pae RNAP-σS holoenzyme, Pae RNAP-σS holoenzyme complexed with SutA, and Pae RNAP-σS transcription initiation complex comprising SutA. The structures show SutA pinches RNAP-β protrusion and facilitates promoter unwinding by wedging RNAP-β lobe open. Our results demonstrate that SutA clears an energetic barrier to facilitate promoter unwinding of RNAP-σS holoenzyme.

Reviews citing this publication (1)

  1. A new twist on PIFE: photoisomerisation-related fluorescence enhancement. Ploetz E, Ambrose B, Barth A, Börner R, Erichson F, Kapanidis AN, Kim HD, Levitus M, Lohman TM, Mazumder A, Rueda DS, Steffen FD, Cordes T, Magennis SW, Lerner E. Methods Appl Fluoresc 12 (2023)

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  2. Structure of the transcription open complex of distinct σI factors. Li J, Zhang H, Li D, Liu YJ, Bayer EA, Cui Q, Feng Y, Zhu P. Nat Commun 14 6455 (2023)
  3. A widely conserved protein Rof inhibits transcription termination factor Rho and promotes Salmonella virulence program. Zhang J, Zhang S, Zhou W, Zhang X, Li G, Li R, Lin X, Chen Z, Liu F, Shen P, Zhou X, Gao Y, Chen Z, Chao Y, Wang C. Nat Commun 15 3187 (2024)
  4. Identification and Structural Modeling of the RNA Polymerase Omega Subunits in Chlamydiae and Other Obligate Intracellular Bacteria. Cheng A, Wan D, Ghatak A, Wang C, Feng D, Fondell JD, Ebright RH, Fan H. mBio 14 e0349922 (2023)
  5. Structural basis of promoter recognition by Staphylococcus aureus RNA polymerase. Yuan L, Liu Q, Xu L, Wu B, Feng Y. Nat Commun 15 4850 (2024)


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