› 7xp5

Electron Microscopy
3.08Å resolution

Cryo-EM structure of a class T GPCR in ligand-free state

Released:
DOI: 10.2210/pdb7xp5/pdb
PDB entry 7xp5 coloured by chain, front view.
PDB entry 7xp5 coloured by chain, side view.
PDB entry 7xp5 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for strychnine activation of human bitter taste receptor TAS2R46.
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science 377 1298-1304 (2022)
PMID: 36108005
Related structures: EMD-33365

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225499 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Endoglucanase H; Taste receptor type 2 member 46 Chain: R
Molecule details ›
Chain: R
Length: 802 amino acids
Theoretical weight: 91.12 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P16218 (Residues: 26-304; Coverage: 30%)
  • Canonical: P59540 (Residues: 2-309; Coverage: 100%)
Gene names: Cthe_1472, TAS2R46, celH
Sequence domains:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 30.58 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 5-389; Coverage: 62%)
  • Best match: P63092-2 (Residues: 5-375)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 366 amino acids
Theoretical weight: 39.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 139 amino acids
Theoretical weight: 15.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.08Å
Relevant EMDB volumes: EMD-33365
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

4 review citations

Bridging structural and cell biology with cryo-electron microscopy.
Nogales et al. (2024)
3 more

1 mention without citation

Activation Profile of TAS2R2, the 26th Human Bitter Taste Receptor.
Lang et al. (2023)