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Crystal structure of T2R-TTL-3a complex

X-ray diffraction
2.81Å resolution
PDB entry 7xr1 coloured by chain, front view.
PDB entry 7xr1 coloured by chain, side view.
PDB entry 7xr1 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero hexamer (preferred)
Entry contents: 4 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero hexamer
Accessible surface area: 81302.89 Å2
Buried surface area: 21192.63 Å2
Dissociation area: 1,118.92 Å2
Dissociation energy (ΔGdiss): -7.16 kcal/mol
Dissociation entropy (TΔSdiss): 14.73 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-236951

Macromolecules

Tubulin alpha-1B chain
Chains: A, C
Length: 450 amino acids
Theoretical weight: 50.04 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-450; Coverage: 100%)
Gene name: TUBA1B
Pfam:
Tubulin alpha-1B chain in PDB entry 7xr1, assembly 1, front view.
Tubulin alpha-1B chain in PDB entry 7xr1, assembly 1, side view.
Tubulin alpha-1B chain in PDB entry 7xr1, assembly 1, top view.
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Tubulin beta chain
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Pfam:
Tubulin beta chain in PDB entry 7xr1, assembly 1, front view.
Tubulin beta chain in PDB entry 7xr1, assembly 1, side view.
Tubulin beta chain in PDB entry 7xr1, assembly 1, top view.
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Stathmin-4
Chain: E
Length: 143 amino acids
Theoretical weight: 16.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63042 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Pfam: Stathmin family
Stathmin-4 in PDB entry 7xr1, assembly 1, front view.
Stathmin-4 in PDB entry 7xr1, assembly 1, side view.
Stathmin-4 in PDB entry 7xr1, assembly 1, top view.
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TTL
Chain: F
Length: 384 amino acids
Theoretical weight: 44.38 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
TTL ligase in PDB entry 7xr1, assembly 1, front view.
TTL ligase in PDB entry 7xr1, assembly 1, side view.
TTL ligase in PDB entry 7xr1, assembly 1, top view.
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