› 7y24

Electron Microscopy
3.25Å resolution

Cryo-EM structure of the octreotide-bound SSTR2-miniGo-scFv16 complex

Released:
DOI: 10.2210/pdb7y24/pdb
PDB entry 7y24 coloured by chain, front view.
PDB entry 7y24 coloured by chain, side view.
PDB entry 7y24 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for the selective G protein signaling of somatostatin receptors.
Chen S, Teng X, Zheng S
Nat Chem Biol (2022)
PMID: 36138141
Related structures: EMD-33585

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-225116 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(o) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 25.06 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P09471 (Residues: 5-57, 182-354; Coverage: 61%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 338 amino acids
Theoretical weight: 37.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 3-340; Coverage: 99%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 56 amino acids
Theoretical weight: 6.16 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 7-62; Coverage: 79%)
Gene name: GNG2
Sequence domains: GGL domain
single Fab chain (svFv16) Chain: S
Molecule details ›
Chain: S
Length: 246 amino acids
Theoretical weight: 26.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Octreotide Chain: C
Molecule details ›
Chain: C
Length: 8 amino acids
Theoretical weight: 1.04 KDa
Source organism: Homo sapiens
Expression system: Not provided
Somatostatin receptor type 2 Chain: E
Molecule details ›
Chain: E
Length: 327 amino acids
Theoretical weight: 36.74 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P30874 (Residues: 1-327; Coverage: 89%)
Gene name: SSTR2
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

No bound ligands
2 modified residues:
Ligand DPN 1 x DPN
Ligand DTR 1 x DTR

Experiments and Validation Details

Entry percentile scores
Resolution: 3.25Å
Relevant EMDB volumes: EMD-33585
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

1 review citation

Allosteric Regulation of G-Protein-Coupled Receptors: From Diversity of Molecular Mechanisms to Multiple Allosteric Sites and Their Ligands.
Shpakov AO. (2023)
8 other articles

1 mention without citation

Identification of a Novel SSTR3 Full Agonist for the Treatment of Nonfunctioning Pituitary Adenomas.
Modena et al. (2023)