› 7yai

Electron Microscopy
3.14Å resolution

CryoEM structure of SPCA1a in E1-Ca-AMPPCP state subclass 3

Released:
DOI: 10.2210/pdb7yai/pdb
PDB entry 7yai coloured by chain, front view.
PDB entry 7yai coloured by chain, side view.
PDB entry 7yai coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures of human SPCA1a reveal the mechanism of Ca2+/Mn2+ transport into the Golgi apparatus.
Chen Z, Watanabe S, Hashida H, Inoue M, Daigaku Y, Kikkawa M, Inaba K
OpenAccess logo Sci Adv 9 eadd9742 (2023)
PMID: 36867705
Related structures: EMD-33713

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225788 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calcium-transporting ATPase type 2C member 1 Chain: A
Molecule details ›
Chain: A
Length: 947 amino acids
Theoretical weight: 103.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P98194 (Residues: 1-919; Coverage: 100%)
Gene names: ATP2C1, HUSSY-28, KIAA1347, PMR1L
Sequence domains:
Nanobody head piece of megabody Chain: C
Molecule details ›
Chain: C
Length: 128 amino acids
Theoretical weight: 13.9 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ACP 1 x ACP
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.14Å
Relevant EMDB volumes: EMD-33713
Expression systems:
  • Homo sapiens
  • Escherichia coli

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Citations

1 review citation

Structures and coordination chemistry of transporters involved in manganese and iron homeostasis.
Ray et al. (2023)
1 other article