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CryoEM structure of Arabidopsis ROS1 in complex with 5mC-dsDNA at 3.1 Angstroms resolution

Electron Microscopy
3.1Å resolution
PDB entry 7yhp coloured by chain, front view.
PDB entry 7yhp coloured by chain, side view.
PDB entry 7yhp coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA glycosylase/AP lyase ROS1, Sex-determining region Y protein and DNA
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Multimeric state: hetero trimer
Accessible surface area: 22327.42 Å2
Buried surface area: 4870.51 Å2
Dissociation area: 2,146.59 Å2
Dissociation energy (ΔGdiss): 26.25 kcal/mol
Dissociation entropy (TΔSdiss): 23.01 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-122334

Macromolecules

DNA glycosylase/AP lyase ROS1; Sex-determining region Y protein
Chain: A
Length: 757 amino acids
Theoretical weight: 86.26 KDa
Source organisms: Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q05066 (Residues: 56-130; Coverage: 37%)
  • Canonical: Q9SJQ6 (Residues: 511-1393; Coverage: 49%)
    • nullnull
Gene names: At2g36490, DML1, F1O11.12, ROS1, SRY, TDF
Pfam:
InterPro:
DNA glycosylase/AP lyase ROS1 in PDB entry 7yhp, assembly 1, front view.
DNA glycosylase/AP lyase ROS1 in PDB entry 7yhp, assembly 1, side view.
DNA glycosylase/AP lyase ROS1 in PDB entry 7yhp, assembly 1, top view.
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DNA chain B
Name: DNA (40-MER)
Representative chains: B
Length: 40 nucleotides
Theoretical weight: 12.34 KDa
DNA (40-MER) in PDB entry 7yhp, assembly 1, front view.
DNA (40-MER) in PDB entry 7yhp, assembly 1, side view.
DNA (40-MER) in PDB entry 7yhp, assembly 1, top view.
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DNA chain C
Name: DNA (40-MER)
Representative chains: C
Length: 40 nucleotides
Theoretical weight: 12.29 KDa
DNA (40-MER) in PDB entry 7yhp, assembly 1, front view.
DNA (40-MER) in PDB entry 7yhp, assembly 1, side view.
DNA (40-MER) in PDB entry 7yhp, assembly 1, top view.
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