› 7yl5

X-ray diffraction
3Å resolution

Cell surface protein YwfG protein complexed with mannose

Released:
DOI: 10.2210/pdb7yl5/pdb
PDB entry 7yl5 coloured by chain, front view.
PDB entry 7yl5 coloured by chain, side view.
PDB entry 7yl5 coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis
Primary publication:
Cell-surface protein YwfG of Lactococcus lactis binds to α-1,2-linked mannose.
Tsuchiya W, Fujimoto Z, Inagaki N, Nakagawa H, Tanaka M, Kimoto-Nira H, Yamazaki T, Suzuki C
PLoS One 18 e0273955 (2023)
PMID: 36602978

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gram-positive cocci surface proteins LPxTG domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 505 amino acids
Theoretical weight: 54.04 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: S6FKX6 (Residues: 28-511; Coverage: 52%)
Gene names: O9U_11750, llmg_2465
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand MAN 3 x MAN
Ligand SO4 25 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: I222
Unit cell:
a: 43.312Å b: 273.164Å c: 322.789Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.227 0.296
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Cell-surface protein YwfG of Lactococcus lactis binds to α-1,2-linked mannose.
Tsuchiya et al. (2023)