› 7yoo

Electron Microscopy
3.11Å resolution

Complex structure of Neuropeptide Y Y2 receptor in complex with NPY and Gi

Released:
DOI: 10.2210/pdb7yoo/pdb
PDB entry 7yoo coloured by chain, front view.
PDB entry 7yoo coloured by chain, side view.
PDB entry 7yoo coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for Y2 receptor-mediated neuropeptide Y and peptide YY signaling.
Kang H, Park C, Choi YK, Bae J, Kwon S, Kim J, Choi C, Seok C, Im W, Choi HJ
Structure 31 44-57.e6 (2023)
PMID: 36525977
Related structures: EMD-33985

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-224952 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Neuropeptide Y Chain: L
Molecule details ›
Chain: L
Length: 36 amino acids
Theoretical weight: 4.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01303 (Residues: 29-64; Coverage: 52%)
Gene name: NPY
Sequence domains: Pancreatic hormone peptide
Neuropeptide Y receptor type 2 Chain: R
Molecule details ›
Chain: R
Length: 391 amino acids
Theoretical weight: 43.78 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P49146 (Residues: 2-381; Coverage: 100%)
Gene name: NPY2R
Sequence domains: 7 transmembrane receptor (rhodopsin family)
single-chain antibody Fv fragment (scFv16) Chain: S
Molecule details ›
Chain: S
Length: 259 amino acids
Theoretical weight: 27.78 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.11Å
Relevant EMDB volumes: EMD-33985
Expression systems:
  • Trichoplusia ni
  • Not provided
  • Spodoptera frugiperda

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