› 7yzg

X-ray diffraction
2.82Å resolution

Crystal structure of the Xenopus FoxH1 bound to the TGTGGATT site

Released:
DOI: 10.2210/pdb7yzg/pdb
PDB entry 7yzg coloured by chain, front view.
PDB entry 7yzg coloured by chain, side view.
PDB entry 7yzg coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
Molecular basis for DNA recognition by the maternal pioneer transcription factor FoxH1.
Pluta R, Aragón E, Prescott NA, Ruiz L, Mees RA, Baginski B, Flood JR, Martin-Malpartida P, Massagué J, David Y, Macias MJ
Nat Commun 13 7279 (2022)
PMID: 36435807

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122356 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein H1 Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 16.29 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P70056 (Residues: 97-236; Coverage: 27%)
Gene names: fast-1, fast1, foxh1, foxh1a
Sequence domains: Forkhead domain
DNA (5'-D(*CP*AP*GP*AP*TP*TP*GP*TP*GP*GP*AP*TP*TP*GP*AP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.99 KDa
Source organism: Xenopus laevis
Expression system: Not provided
DNA (5'-D(*CP*TP*CP*AP*AP*TP*CP*CP*AP*CP*AP*AP*TP*CP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.8 KDa
Source organism: Xenopus laevis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 46.304Å b: 78.726Å c: 103.253Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

TGF-β signaling in health and disease.
Massagué et al. (2023)
1 other article