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Crystal structure of the Xenopus FoxH1 bound to the TGTGGATT site

X-ray diffraction
2.82Å resolution
PDB entry 7yzg coloured by chain, front view.
PDB entry 7yzg coloured by chain, side view.
PDB entry 7yzg coloured by chain, top view.
Source organism: Xenopus laevis
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Forkhead box protein H1 and DNA
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Multimeric state: hetero trimer
Accessible surface area: 11425.93 Å2
Buried surface area: 3987.27 Å2
Dissociation area: 1,279.06 Å2
Dissociation energy (ΔGdiss): 14.06 kcal/mol
Dissociation entropy (TΔSdiss): 11.27 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-270259

Macromolecules

Forkhead box protein H1
Chain: A
Length: 140 amino acids
Theoretical weight: 16.29 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P70056 (Residues: 97-236; Coverage: 27%)
Gene names: fast-1, fast1, foxh1, foxh1a
Pfam: Forkhead domain
InterPro:
Forkhead box protein H1 in PDB entry 7yzg, assembly 1, front view.
Forkhead box protein H1 in PDB entry 7yzg, assembly 1, side view.
Forkhead box protein H1 in PDB entry 7yzg, assembly 1, top view.
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DNA chain B
Name: DNA (5'-D(*CP*AP*GP*AP*TP*TP*GP*TP*GP*GP*AP*TP*TP*GP*AP*G)-3')
Representative chains: B
Source organism: Xenopus laevis [8355]
Expression system: Not provided
Length: 16 nucleotides
Theoretical weight: 4.99 KDa
DNA (5'-D(*CP*AP*GP*AP*TP*TP*GP*TP*GP*GP*AP*TP*TP*GP*AP*G)-3') in PDB entry 7yzg, assembly 1, front view.
DNA (5'-D(*CP*AP*GP*AP*TP*TP*GP*TP*GP*GP*AP*TP*TP*GP*AP*G)-3') in PDB entry 7yzg, assembly 1, side view.
DNA (5'-D(*CP*AP*GP*AP*TP*TP*GP*TP*GP*GP*AP*TP*TP*GP*AP*G)-3') in PDB entry 7yzg, assembly 1, top view.
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DNA chain C
Name: DNA (5'-D(*CP*TP*CP*AP*AP*TP*CP*CP*AP*CP*AP*AP*TP*CP*TP*G)-3')
Representative chains: C
Source organism: Xenopus laevis [8355]
Expression system: Not provided
Length: 16 nucleotides
Theoretical weight: 4.8 KDa
DNA (5'-D(*CP*TP*CP*AP*AP*TP*CP*CP*AP*CP*AP*AP*TP*CP*TP*G)-3') in PDB entry 7yzg, assembly 1, front view.
DNA (5'-D(*CP*TP*CP*AP*AP*TP*CP*CP*AP*CP*AP*AP*TP*CP*TP*G)-3') in PDB entry 7yzg, assembly 1, side view.
DNA (5'-D(*CP*TP*CP*AP*AP*TP*CP*CP*AP*CP*AP*AP*TP*CP*TP*G)-3') in PDB entry 7yzg, assembly 1, top view.
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