Assemblies
Assembly Name:
DNA-directed RNA polymerase III complex and Integrase
Multimeric state:
hetero nonadecamer
Accessible surface area:
202160.71 Å2
Buried surface area:
100631.37 Å2
Dissociation area:
514.03
Å2
Dissociation energy (ΔGdiss):
1.6
kcal/mol
Dissociation entropy (TΔSdiss):
7.06
kcal/mol
Symmetry number:
1
PDBe Complex ID:
PDB-CPX-228488
Macromolecules
Chain: A
Length: 1460 amino acids
Theoretical weight: 162.52 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam:
Length: 1460 amino acids
Theoretical weight: 162.52 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P04051 (Residues: 1-1460; Coverage: 100%)
Pfam:
- RNA polymerase Rpb1, domain 1
- RNA polymerase Rpb1, domain 2
- RNA polymerase Rpb1, domain 3
- RNA polymerase Rpb1, domain 4
- RNA polymerase Rpb1, domain 5
- RNA polymerase Rpb1, clamp domain superfamily
- RNA polymerase Rpb1, domain 1
- DNA-directed RNA polymerase, subunit beta-prime
- DNA-directed RNA polymerase III subunit RPC1, N-terminal
- RNA polymerase, N-terminal
- RNA polymerase, alpha subunit
- RNA polymerase Rpb1, domain 3
- RNA polymerase Rpb1, domain 3 superfamily
- RNA polymerase Rpb1, funnel domain superfamily
- RNA polymerase Rpb1, domain 4
- RNA polymerase Rpb1, domain 5
- DNA-directed RNA polymerase III subunit RPC1, C-terminal
Chain: B
Length: 1149 amino acids
Theoretical weight: 129.63 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam:
Length: 1149 amino acids
Theoretical weight: 129.63 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P22276 (Residues: 1-1149; Coverage: 100%)
Pfam:
- RNA polymerase beta subunit
- RNA polymerase Rpb2, domain 2
- RNA polymerase Rpb2, domain 3
- RNA polymerase Rpb2, domain 4
- RNA polymerase Rpb2, domain 5
- RNA polymerase Rpb2, domain 6
- RNA polymerase Rpb2, domain 7
- DNA-directed RNA polymerase, subunit 2
- RNA polymerase, beta subunit, protrusion
- RNA polymerase Rpb2, domain 2
- RNA polymerase Rpb2, domain 2 superfamily
- RNA polymerase Rpb2, domain 3
- RNA polymerase Rpb2, domain 4
- RNA polymerase Rpb2, domain 5
- DNA-directed RNA polymerase, subunit 2, hybrid-binding domain
- DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily
- RNA polymerase Rpb2, OB-fold
- RNA polymerase, beta subunit, conserved site
- RNA polymerase Rpb2, domain 7
Chain: C
Length: 335 amino acids
Theoretical weight: 37.73 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam:
InterPro:
Length: 335 amino acids
Theoretical weight: 37.73 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P07703 (Residues: 1-335; Coverage: 100%)
Pfam:
InterPro:
- RNA polymerase, RBP11-like subunit
- DNA-directed RNA polymerases I and III subunit AC40
- DNA-directed RNA polymerase subunit Rpo3/Rpb3/RPAC1
- DNA-directed RNA polymerase, RpoA/D/Rpb3-type
- DNA-directed RNA polymerase, 30-40kDa subunit, conserved site
- DNA-directed RNA polymerase, insert domain superfamily
- DNA-directed RNA polymerase, insert domain
Chain: D
Length: 161 amino acids
Theoretical weight: 18.62 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam: RNA polymerase Rpb4
InterPro:
Length: 161 amino acids
Theoretical weight: 18.62 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P47076 (Residues: 1-161; Coverage: 100%)
Pfam: RNA polymerase Rpb4
InterPro:
Chain: F
Length: 155 amino acids
Theoretical weight: 17.93 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam: RNA polymerase Rpb6
InterPro:
Length: 155 amino acids
Theoretical weight: 17.93 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P20435 (Residues: 1-155; Coverage: 100%)
Pfam: RNA polymerase Rpb6
InterPro:
Chain: H
Length: 146 amino acids
Theoretical weight: 16.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam: RNA polymerase Rpb8
InterPro:
Length: 146 amino acids
Theoretical weight: 16.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P20436 (Residues: 1-146; Coverage: 100%)
Pfam: RNA polymerase Rpb8
InterPro:
Chain: I
Length: 110 amino acids
Theoretical weight: 12.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam:
InterPro:
Length: 110 amino acids
Theoretical weight: 12.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
Q04307 (Residues: 1-110; Coverage: 100%)
Pfam:
InterPro:
Chain: J
Length: 70 amino acids
Theoretical weight: 8.29 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam: RNA polymerases N / 8 kDa subunit
InterPro:
Length: 70 amino acids
Theoretical weight: 8.29 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P22139 (Residues: 1-70; Coverage: 100%)
Pfam: RNA polymerases N / 8 kDa subunit
InterPro:
Chain: K
Length: 142 amino acids
Theoretical weight: 16.17 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam: RNA polymerase Rpb3/Rpb11 dimerisation domain
InterPro:
Length: 142 amino acids
Theoretical weight: 16.17 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P28000 (Residues: 1-142; Coverage: 100%)
Pfam: RNA polymerase Rpb3/Rpb11 dimerisation domain
InterPro:
Chain: L
Length: 70 amino acids
Theoretical weight: 7.29 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam: DNA directed RNA polymerase, 7 kDa subunit
InterPro:
Length: 70 amino acids
Theoretical weight: 7.29 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P40422 (Residues: 26-70; Coverage: 64%)
Pfam: DNA directed RNA polymerase, 7 kDa subunit
InterPro:
Chain: M
Length: 282 amino acids
Theoretical weight: 32.18 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam: RPC5 protein
InterPro: DNA-directed RNA polymerase III subunit Rpc5
Length: 282 amino acids
Theoretical weight: 32.18 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P36121 (Residues: 1-282; Coverage: 100%)
Pfam: RPC5 protein
InterPro: DNA-directed RNA polymerase III subunit Rpc5
Chain: N
Length: 422 amino acids
Theoretical weight: 42.12 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam: RNA polymerase III RPC4
InterPro: DNA-directed RNA polymerase III subunit RPC4
Length: 422 amino acids
Theoretical weight: 42.12 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P25441 (Residues: 195-422; Coverage: 54%)
Pfam: RNA polymerase III RPC4
InterPro: DNA-directed RNA polymerase III subunit RPC4
Chain: O
Length: 654 amino acids
Theoretical weight: 74.11 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam:
Length: 654 amino acids
Theoretical weight: 74.11 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P32349 (Residues: 1-654; Coverage: 100%)
Pfam:
- RNA polymerase III subunit RPC82 helix-turn-helix domain
- RNA polymerase III subunit RPC82
- POLR3C, C-terminal winged-helix domain
- DNA-directed RNA polymerase III subunit RPC3, helical hairpin domain
Chain: P
Length: 317 amino acids
Theoretical weight: 36.17 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam: RNA polymerase Rpc34 subunit
InterPro:
Length: 317 amino acids
Theoretical weight: 36.17 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P32910 (Residues: 1-317; Coverage: 100%)
Pfam: RNA polymerase Rpc34 subunit
InterPro:
Chain: Q
Length: 268 amino acids
Theoretical weight: 29.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Pfam: DNA-directed RNA polymerase III subunit Rpc31
InterPro: DNA-directed RNA polymerase III, subunit Rpc31
Length: 268 amino acids
Theoretical weight: 29.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical:
P17890 (Residues: 1-251; Coverage: 100%)
Pfam: DNA-directed RNA polymerase III subunit Rpc31
InterPro: DNA-directed RNA polymerase III, subunit Rpc31
Chain: W
Length: 635 amino acids
Theoretical weight: 71.61 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam: Integrase core domain
InterPro:
Length: 635 amino acids
Theoretical weight: 71.61 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q12414 (Residues: 583-1217; Coverage: 36%)
Pfam: Integrase core domain
InterPro:
Chain: X
Length: 13 amino acids
Theoretical weight: 1.12 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
Length: 13 amino acids
Theoretical weight: 1.12 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C