› 7z3q

X-ray diffraction
3.62Å resolution

Crystal structure of the human leptin:LepR-CRH2 encounter complex to 3.6 A resolution.

Released:
DOI: 10.2210/pdb7z3q/pdb
PDB entry 7z3q coloured by chain, front view.
PDB entry 7z3q coloured by chain, side view.
PDB entry 7z3q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of receptor assembly via the pleiotropic adipokine Leptin.
Tsirigotaki A, Dansercoer A, Verschueren KHG, Marković I, Pollmann C, Hafer M, Felix J, Birck C, Van Putte W, Catteeuw D, Tavernier J, Fernando Bazan J, Piehler J, Savvides SN, Verstraete K
Nat Struct Mol Biol (2023)
PMID: 36959263

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273702 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Leptin Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 171 amino acids
Theoretical weight: 18.61 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P41159 (Residues: 22-167; Coverage: 100%)
Gene names: LEP, OB, OBS
Sequence domains: Leptin
Leptin receptor Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 232 amino acids
Theoretical weight: 26.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P48357 (Residues: 428-635; Coverage: 18%)
Gene names: DB, LEPR, OBR
Sequence domains: Obesity receptor immunoglobulin like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C222
Unit cell:
a: 156.197Å b: 156.644Å c: 95.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.277 0.275 0.305
Expression system: Homo sapiens

Quick links

Citations

4 citation in other articles

Structural insights into the mechanism of leptin receptor activation.
Saxton et al. (2023)
3 more