7zli

Electron Microscopy
2.99Å resolution

Cryo-EM structure of C-mannosyltransferase CeDPY19, in complex with Dol25-P-Man and bound to CMT2-Fab and anti-Fab nanobody

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-225601 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
CMT2-Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 236 amino acids
Theoretical weight: 25 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Anti-Fab nanobody Chain: K
Molecule details ›
Chain: K
Length: 123 amino acids
Theoretical weight: 13.39 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.24 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z3Y4 (Residues: 23-236; Coverage: 100%)
Sequence domains:
C-mannosyltransferase dpy-19 Chain: A
Molecule details ›
Chain: A
Length: 707 amino acids
Theoretical weight: 80.89 KDa
Source organism: Caenorhabditis elegans
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P34413 (Residues: 1-683; Coverage: 100%)
Gene names: F22B7.10, dpy-19
Sequence domains: Q-cell neuroblast polarisation

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.99Å
Relevant EMDB volumes: EMD-14781
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda