› 8fxs

X-ray diffraction
3.15Å resolution

Crystal structure of human pro-TGF-beta2 in complex with Nb9

Released:
DOI: 10.2210/pdb8fxs/pdb
PDB entry 8fxs coloured by chain, front view.
PDB entry 8fxs coloured by chain, side view.
PDB entry 8fxs coloured by chain, top view.
Source organisms:
Primary publication:
A specialized integrin-binding motif enables proTGF-β2 activation by integrin αVβ6 but not αVβ8.
Le VQ, Zhao B, Ramesh S, Toohey C, DeCosta A, Mintseris J, Liu X, Gygi S, Springer TA
Proc Natl Acad Sci U S A 120 e2304874120 (2023)
PMID: 37279271

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225765 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transforming growth factor beta-2 proprotein Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 45.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P61812 (Residues: 21-414; Coverage: 99%)
Gene name: TGFB2
Sequence domains:
Nanobody clone 9 Chains: D, E
Molecule details ›
Chains: D, E
Length: 124 amino acids
Theoretical weight: 13.8 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P21
Unit cell:
a: 69.683Å b: 89.554Å c: 89.74Å
α: 90° β: 95° γ: 90°
R-values:
R R work R free
0.255 0.249 0.301
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

TGF-β signaling in health and disease.
Massagué et al. (2023)