› 8fxv

X-ray diffraction
2.2Å resolution

Crystal structure of human proTGF-beta2 in complex with Nb18

Released:
DOI: 10.2210/pdb8fxv/pdb
PDB entry 8fxv coloured by chain, front view.
PDB entry 8fxv coloured by chain, side view.
PDB entry 8fxv coloured by chain, top view.
Source organisms:
Primary publication:
A specialized integrin-binding motif enables proTGF-β2 activation by integrin αVβ6 but not αVβ8.
Le VQ, Zhao B, Ramesh S, Toohey C, DeCosta A, Mintseris J, Liu X, Gygi S, Springer TA
OpenAccess logo Proc Natl Acad Sci U S A 120 e2304874120 (2023)
PMID: 37279271

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225766 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transforming growth factor beta-2 proprotein Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 45.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P61812 (Residues: 21-414; Coverage: 99%)
Gene name: TGFB2
Sequence domains:
Nanobody clone 18 Chain: B
Molecule details ›
Chain: B
Length: 124 amino acids
Theoretical weight: 13.52 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Unit cell:
a: 70.75Å b: 78.16Å c: 88.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.227 0.249
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

TGF-β signaling in health and disease.
Massagué et al. (2023)