› 8szh

Electron Microscopy
3.1Å resolution

Cryo-EM structure of cinacalcet-bound human calcium-sensing receptor CaSR-Gi complex in lipid nanodiscs

Released:
DOI: 10.2210/pdb8szh/pdb
PDB entry 8szh coloured by chain, front view.
PDB entry 8szh coloured by chain, side view.
PDB entry 8szh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric modulation and G-protein selectivity of the Ca2+-sensing receptor.
He F, Wu CG, Gao Y, Rahman SN, Zaoralová M, Papasergi-Scott MM, Gu TJ, Robertson MJ, Seven AB, Li L, Mathiesen JM, Skiniotis G
Nature (2024)
PMID: 38326620
Related structures: EMD-40916

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-279563 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Extracellular calcium-sensing receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 886 amino acids
Theoretical weight: 99.93 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41180 (Residues: 19-894; Coverage: 83%)
Gene names: CASR, GPRC2A, PCAR1
Sequence domains:
Guanine nucleotide-binding protein G(i) subunit alpha-3 Chain: C
Molecule details ›
Chain: C
Length: 354 amino acids
Theoretical weight: 40.59 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08754 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI3
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: D
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: E

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
8 bound ligands:
Ligand YP4 2 x YP4
Ligand PCW 2 x PCW
Ligand NAG 3 x NAG
Ligand CA 4 x CA
Ligand TRP 2 x TRP
Ligand PO4 2 x PO4
Ligand SPM 4 x SPM
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.1Å
Relevant EMDB volumes: EMD-40916
Expression system: Spodoptera frugiperda

Quick links

Citations

2 citation in other articles

Control of G protein-coupled receptor function via membrane-interacting intrinsically disordered C-terminal domains.
Mancinelli et al. (2024)
1 more

3 mentions without citation

Molecular regulation of calcium-sensing receptor (CaSR)-mediated signaling.
Tian et al. (2024)
2 more