› 8ajy

X-ray diffraction
1.71Å resolution

Ruminococcus flavefaciens Cohesin-Dockerin structure: dockerin from ScaH adaptor scaffoldin in complex with the cohesin from ScaE anchoring scaffoldin

Released:
DOI: 10.2210/pdb8ajy/pdb
PDB entry 8ajy coloured by chain, front view.
PDB entry 8ajy coloured by chain, side view.
PDB entry 8ajy coloured by chain, top view.
Source organism: Ruminococcus flavefaciens FD-1
Primary publication:
Structure-function studies can improve binding affinity of cohesin-dockerin interactions for multi-protein assemblies.
Duarte M, Alves VD, Correia M, Caseiro C, Ferreira LMA, Romão MJ, Carvalho AL, Najmudin S, Bayer EA, Fontes CMGA, Bule P
Int J Biol Macromol (2022)
PMID: 36252630

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106046 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell-wall anchoring protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 203 amino acids
Theoretical weight: 21.64 KDa
Source organism: Ruminococcus flavefaciens FD-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0AEF6 (Residues: 30-231; Coverage: 67%)
Gene name: scaE
Dockerin from ScaH Chains: B, D
Molecule details ›
Chains: B, D
Length: 112 amino acids
Theoretical weight: 12.18 KDa
Source organism: Ruminococcus flavefaciens FD-1
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand CA 6 x CA
Ligand GOL 2 x GOL
Ligand SO4 13 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 40.81Å b: 120Å c: 65.95Å
α: 90° β: 97.77° γ: 90°
R-values:
R R work R free
0.196 0.194 0.237
Expression system: Escherichia coli BL21(DE3)

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