Structure analysis

Ruminococcus flavefaciens Cohesin-Dockerin structure: dockerin from ScaH adaptor scaffoldin in complex with the cohesin from ScaE anchoring scaffoldin

X-ray diffraction
1.71Å resolution
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

Assemblies

Assembly 1
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Multimeric state: hetero dimer
Accessible surface area: 12932.78 Å2
Buried surface area: 3583.02 Å2
Dissociation area: 950.11 Å2
Dissociation energy (ΔGdiss): 8.94 kcal/mol
Dissociation entropy (TΔSdiss): 11.14 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-269750
Assembly 2 (preferred)
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Multimeric state: hetero dimer
Accessible surface area: 12662.09 Å2
Buried surface area: 3005.61 Å2
Dissociation area: 957.28 Å2
Dissociation energy (ΔGdiss): 8.59 kcal/mol
Dissociation entropy (TΔSdiss): 11.1 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-269750

Macromolecules

Chains: A, C
Length: 203 amino acids
Theoretical weight: 21.64 KDa
Source organism: Ruminococcus flavefaciens FD-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0AEF6 (Residues: 30-231; Coverage: 67%)
Gene name: scaE
InterPro: CBM2/CBM3, carbohydrate-binding domain superfamily

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Chains: B, D
Length: 112 amino acids
Theoretical weight: 12.18 KDa
Source organism: Ruminococcus flavefaciens FD-1
Expression system: Escherichia coli BL21(DE3)
InterPro:

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