8ake

X-ray diffraction
1.82Å resolution

Human Sirt6 in complex with ADP-ribose and fragment nicotinamide

Released:
Source organism: Homo sapiens
Entry authors: You W, Steegborn C

Function and Biology Details

Reaction catalysed:
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-185425 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent protein deacylase sirtuin-6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 33.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N6T7 (Residues: 13-308; Coverage: 83%)
Gene names: SIR2L6, SIRT6
Sequence domains: Sir2 family

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P63
Unit cell:
a: 91.106Å b: 91.106Å c: 143.428Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.224
Expression system: Escherichia coli