› 8aly

Electron Microscopy
2.98Å resolution

Cryo-EM structure of human tankyrase 2 SAM-PARP filament (G1032W mutant)

Released:
DOI: 10.2210/pdb8aly/pdb
PDB entry 8aly coloured by chain, front view.
PDB entry 8aly coloured by chain, side view.
PDB entry 8aly coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of tankyrase activation by polymerization.
Pillay N, Mariotti L, Zaleska M, Inian O, Jessop M, Hibbs S, Desfosses A, Hopkins PCR, Templeton CM, Beuron F, Morris EP, Guettler S
OpenAccess logo Nature 612 162-169 (2022)
PMID: 36418402
Related structures: EMD-15520

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo icosamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190554 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly [ADP-ribose] polymerase tankyrase-2 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Length: 299 amino acids
Theoretical weight: 34.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H2K2 (Residues: 867-1162; Coverage: 25%)
Gene names: PARP5B, TANK2, TNKL, TNKS2
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 20 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.98Å
Relevant EMDB volumes: EMD-15520
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

ADP-ribosylation from molecular mechanisms to therapeutic implications.
Suskiewicz et al. (2023)
4 more

1 mention without citation

PDB NextGen Archive: centralizing access to integrated annotations and enriched structural information by the Worldwide Protein Data Bank.
Choudhary et al. (2024)