Structure analysis

Cryo-EM structure of the proline-rich antimicrobial peptide drosocin bound to the elongating ribosome

Electron Microscopy
2.8Å resolution
Assembly composition:
hetero 56-mer (preferred)
Entry contents: 50 distinct polypeptide molecules
6 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 56-mer
Accessible surface area: 719987.46 Å2
Buried surface area: 316205.12 Å2
Dissociation area: 650.35 Å2
Dissociation energy (ΔGdiss): 0.07 kcal/mol
Dissociation entropy (TΔSdiss): 10.08 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-279464

Macromolecules

Chain: 1
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7P5 (Residues: 1-46; Coverage: 100%)
Gene names: JW3680, b3703, rimA, rpmH, ssaF
Pfam: Ribosomal protein L34
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Chain: 3
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7Q6 (Residues: 1-38; Coverage: 100%)
Gene names: JW3261, b3299, rpmJ
Pfam: Ribosomal protein L36
InterPro:

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Chain: I
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7W7 (Residues: 1-130; Coverage: 100%)
Gene names: JW3268, b3306, rpsH
Pfam: Ribosomal protein S8
InterPro:

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Chain: M
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7S3 (Residues: 1-124; Coverage: 100%)
Gene names: JW3304, b3342, rpsL, strA
Pfam: Ribosomal protein S12/S23
InterPro:

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Chain: O
Length: 101 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0AG59 (Residues: 1-101; Coverage: 100%)
Gene names: JW3269, b3307, rpsN
Pfam: Ribosomal protein S14p/S29e
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Chain: P
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Gene names: JW3134, b3165, rpsO, secC
Pfam: Ribosomal protein S15
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Chain: Q
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Pfam: Ribosomal protein S16
InterPro:

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Chain: R
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0AG63 (Residues: 1-84; Coverage: 100%)
Gene names: JW3273, b3311, neaA, rpsQ
Pfam: Ribosomal protein S17
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Chain: S
Length: 75 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7T7 (Residues: 1-75; Coverage: 100%)
Gene names: JW4160, b4202, rpsR
Pfam: Ribosomal protein S18
InterPro:

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Chain: U
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7U7 (Residues: 1-87; Coverage: 100%)
Gene names: JW0022, b0023, rpsT
Pfam: Ribosomal protein S20
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Chain: V
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P68679 (Residues: 1-71; Coverage: 100%)
Gene names: JW3037, b3065, rpsU
Pfam: Ribosomal protein S21
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Chain: e
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P60723 (Residues: 1-201; Coverage: 100%)
Gene names: JW3281, b3319, eryA, rplD
Pfam: Ribosomal protein L4/L1 family
InterPro:

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Chain: m
Length: 127 amino acids
Theoretical weight: 14.39 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0AG44 (Residues: 1-127; Coverage: 100%)
Gene names: JW3256, b3294, rplQ
Pfam: Ribosomal protein L17
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Chain: n
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0C018 (Residues: 1-117; Coverage: 100%)
Gene names: JW3266, b3304, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
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Chain: o
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7K6 (Residues: 1-115; Coverage: 100%)
Gene names: JW2587, b2606, rplS
Pfam: Ribosomal protein L19
InterPro:

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Chain: p
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7L3 (Residues: 1-118; Coverage: 100%)
Gene names: JW1706, b1716, pdzA, rplT
Pfam: Ribosomal protein L20
InterPro:

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Chain: v
Length: 85 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7L8 (Residues: 1-85; Coverage: 100%)
Gene names: JW3152, b3185, rpmA
Pfam: Ribosomal L27 protein
InterPro:

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Chain: x
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7M6 (Residues: 1-63; Coverage: 100%)
Gene names: JW3274, b3312, rpmC
Pfam: Ribosomal L29 protein
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Chain: z
Length: 57 amino acids
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli BW25113
UniProt:
  • Canonical: P0A7N4 (Residues: 1-57; Coverage: 100%)
Gene names: JW1075, b1089, rpmF
Pfam: Ribosomal L32p protein family
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Chain: A
Length: 19 amino acids
Theoretical weight: 2.2 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BW25113
UniProt:
  • Canonical: P36193 (Residues: 22-40; Coverage: 42%)
Gene names: CG10816, Dro

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Name: 16S ribosomal RNA
Representative chains: B
Length: 1534 nucleotides
Theoretical weight: 497.4 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: 23S ribosomal RNA
Representative chains: a
Length: 2903 nucleotides
Theoretical weight: 941.48 KDa
Rfam: Bacterial large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: b
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Rfam: 5S ribosomal RNA

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Name: Messenger RNA
Representative chains: X
Length: 12 nucleotides
Theoretical weight: 3.82 KDa

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Name: Initiator tRNA
Representative chains: Y
Length: 76 nucleotides
Theoretical weight: 24.54 KDa
Rfam: tRNA

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Name: Leucine tRNA
Representative chains: 8
Length: 85 nucleotides
Theoretical weight: 27.42 KDa
Rfam: tRNA

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