Structure analysis

Cryo-EM structure of the plant 60S subunit

Electron Microscopy
2.14Å resolution
Source organism: Nicotiana tabacum
Assembly composition:
hetero 46-mer (preferred)
Entry contents: 43 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 46-mer
Accessible surface area: 605774.22 Å2
Buried surface area: 492248.58 Å2
Dissociation area: 244.51 Å2
Dissociation energy (ΔGdiss): -4.34 kcal/mol
Dissociation entropy (TΔSdiss): 9.98 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-268973

Macromolecules

Chain: D
Length: 206 amino acids
Theoretical weight: 23.6 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4CSN1 (Residues: 1-206; Coverage: 100%)
Gene name: LOC107822051
Pfam: Ribosomal protein L13e

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Chain: E
Length: 134 amino acids
Theoretical weight: 15.44 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4A807 (Residues: 1-134; Coverage: 100%)
Gene name: LOC107794709
Pfam: Ribosomal protein L14

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Chain: F
Length: 204 amino acids
Theoretical weight: 24.23 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4D5P7 (Residues: 1-204; Coverage: 100%)
Gene name: LOC107826284
Pfam: Ribosomal L15

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Chain: G
Length: 187 amino acids
Theoretical weight: 20.91 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4DF55 (Residues: 1-187; Coverage: 100%)
Gene names: LOC107759208, LOC107829149
Pfam: Ribosomal protein 60S L18 and 50S L18e

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Chain: H
Length: 214 amino acids
Theoretical weight: 25.06 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3X0I4 (Residues: 1-214; Coverage: 100%)
Gene name: LOC107759916
Pfam: Ribosomal protein L19e

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Chain: I
Length: 178 amino acids
Theoretical weight: 21.51 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4CP52 (Residues: 1-178; Coverage: 100%)
Gene names: LOC107775758, LOC107818966, LOC107820975, LOC107821623
Pfam: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A

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Chain: J
Length: 164 amino acids
Theoretical weight: 18.72 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3XCW6 (Residues: 1-164; Coverage: 100%)
Gene name: LOC107763612
Pfam: Ribosomal protein L21e

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Chain: K
Length: 127 amino acids
Theoretical weight: 14.28 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4BSU3 (Residues: 1-127; Coverage: 100%)
Gene name: LOC107811461
Pfam: Ribosomal L22e protein family

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Chain: L
Length: 164 amino acids
Theoretical weight: 18.61 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4BHF2 (Residues: 1-164; Coverage: 100%)
Gene names: LOC107781546, LOC107808292
Pfam: Ribosomal protein L24e

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Chain: M
Length: 135 amino acids
Theoretical weight: 15.72 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4AEL0 (Residues: 1-135; Coverage: 100%)
Gene name: LOC107796761
Pfam:

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Chain: N
Length: 143 amino acids
Theoretical weight: 15.95 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4D1U6 (Residues: 1-143; Coverage: 100%)
Gene name: LOC107824985
Pfam: Ribosomal L28e protein family

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Chain: O
Length: 61 amino acids
Theoretical weight: 7.13 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3YR12 (Residues: 1-61; Coverage: 100%)
Gene name: LOC107778781
Pfam: Ribosomal L29e protein family

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Chain: P
Length: 113 amino acids
Theoretical weight: 12.42 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3WZ09 (Residues: 1-113; Coverage: 100%)
Gene names: LOC107759448, LOC107771134
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

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Chain: Q
Length: 120 amino acids
Theoretical weight: 13.89 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3Y3V5 (Residues: 1-120; Coverage: 100%)
Gene names: LOC107771890, LOC107792531
Pfam: Ribosomal protein L31e

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Chain: R
Length: 133 amino acids
Theoretical weight: 15.67 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3Z2X4 (Residues: 1-133; Coverage: 100%)
Gene names: LOC107782359, LOC107785823
Pfam: Ribosomal protein L32

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Chain: S
Length: 112 amino acids
Theoretical weight: 12.96 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3XU79 (Residues: 1-112; Coverage: 100%)
Gene names: LOC107768641, LOC107779239, LOC107815581
Pfam: Ribosomal protein L35Ae

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Chain: T
Length: 120 amino acids
Theoretical weight: 13.79 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: P41098 (Residues: 1-120; Coverage: 100%)
Gene name: RPL34
Pfam: Ribosomal protein L34e

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Chain: U
Length: 110 amino acids
Theoretical weight: 12.36 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4D2I2 (Residues: 1-110; Coverage: 100%)
Gene name: LOC107825165
Pfam: Ribosomal protein L36e

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Chain: V
Length: 95 amino acids
Theoretical weight: 10.88 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4DN51 (Residues: 1-95; Coverage: 100%)
Gene names: LOC107784204, LOC107831585, LOC107832335
Pfam: Ribosomal protein L37e

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Chain: W
Length: 69 amino acids
Theoretical weight: 8.1 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4C4G8 (Residues: 1-69; Coverage: 100%)
Gene names: LOC107767504, LOC107815038
Pfam: Ribosomal L38e protein family

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Chain: X
Length: 51 amino acids
Theoretical weight: 6.5 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3YR32 (Residues: 1-51; Coverage: 100%)
Gene names: LOC107774666, LOC107778797, LOC107781601, LOC107813331, LOC107814522
Pfam: Ribosomal L39 protein

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Chain: Y
Length: 128 amino acids
Theoretical weight: 14.72 KDa
Source organism: Nicotiana tabacum

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Chain: p
Length: 25 amino acids
Theoretical weight: 3.45 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: P62121 (Residues: 1-25; Coverage: 100%)
Gene name: RPL41
Pfam: Ribosomal protein L41

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Chain: Z
Length: 105 amino acids
Theoretical weight: 12.04 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4AVP7 (Residues: 1-105; Coverage: 100%)
Gene names: LOC107761379, LOC107767162, LOC107792576, LOC107795073, LOC107801799, LOC107802399, LOC107818115
Pfam: Ribosomal protein L44

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Chain: a
Length: 92 amino acids
Theoretical weight: 10.28 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3X5Y9 (Residues: 1-92; Coverage: 100%)
Gene names: LOC107761416, LOC107766434, LOC107769002, LOC107772948, LOC107783422, LOC107787560, LOC107803180, LOC107806598, LOC107814281
Pfam: Ribosomal L37ae protein family

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Chain: b
Length: 230 amino acids
Theoretical weight: 25.54 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4B0G2 (Residues: 1-230; Coverage: 100%)
Gene name: LOC107803255
Pfam:

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Chain: c
Length: 258 amino acids
Theoretical weight: 29.43 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4D668 (Residues: 1-258; Coverage: 100%)
Gene name: LOC107826327
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

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Chain: d
Length: 206 amino acids
Theoretical weight: 23.53 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4DN49 (Residues: 1-206; Coverage: 100%)
Gene name: LOC107831576
Pfam: Ribosomal protein L13

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Chain: e
Length: 140 amino acids
Theoretical weight: 15.04 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3XSQ1 (Residues: 1-140; Coverage: 100%)
Gene names: LOC107761645, LOC107768301, LOC107774008, LOC107812746
Pfam: Ribosomal protein L14p/L23e

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Chain: f
Length: 148 amino acids
Theoretical weight: 16.58 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4BFD5 (Residues: 1-148; Coverage: 100%)
Gene name: LOC107807693
Pfam: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

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Chain: g
Length: 221 amino acids
Theoretical weight: 25.01 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3Z2D7 (Residues: 1-221; Coverage: 100%)
Gene name: LOC107782233
Pfam: Ribosomal protein L16p/L10e

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Chain: h
Length: 301 amino acids
Theoretical weight: 34.63 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3XE16 (Residues: 1-301; Coverage: 100%)
Gene name: LOC107764090
Pfam:

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Chain: j
Length: 175 amino acids
Theoretical weight: 20.14 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4CCH0 (Residues: 1-175; Coverage: 100%)
Gene name: LOC107817504
Pfam: Ribosomal protein L22p/L17e

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Chain: k
Length: 154 amino acids
Theoretical weight: 17.32 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: Q07761 (Residues: 1-154; Coverage: 100%)
Gene names: RPL23A, RPL25
Pfam:

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Chain: m
Length: 146 amino acids
Theoretical weight: 16.75 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4DPM5 (Residues: 1-146; Coverage: 100%)
Gene name: LOC107831809
Pfam:

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Chain: n
Length: 123 amino acids
Theoretical weight: 14.35 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3WY31 (Residues: 1-123; Coverage: 100%)
Gene name: LOC107759229
Pfam: Ribosomal L29 protein

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Chain: o
Length: 260 amino acids
Theoretical weight: 28.22 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3XXQ5 (Residues: 1-260; Coverage: 100%)
Gene names: LOC107769978, LOC107827527
Pfam:

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Chain: q
Length: 242 amino acids
Theoretical weight: 28.15 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4A5Z2 (Residues: 1-242; Coverage: 100%)
Gene name: LOC107794124
Pfam:

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Chain: r
Length: 389 amino acids
Theoretical weight: 44.51 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3ZLI9 (Residues: 1-389; Coverage: 100%)
Gene name: LOC107788247
Pfam: Ribosomal protein L3

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Chain: s
Length: 405 amino acids
Theoretical weight: 44.71 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3X0M5 (Residues: 1-405; Coverage: 100%)
Gene name: LOC107759872
Pfam:

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Chain: t
Length: 181 amino acids
Theoretical weight: 20.79 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S3XAW1 (Residues: 1-181; Coverage: 100%)
Gene name: LOC107763118
Pfam:

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Chain: u
Length: 194 amino acids
Theoretical weight: 22.06 KDa
Source organism: Nicotiana tabacum
UniProt:
  • Canonical: A0A1S4C068 (Residues: 1-194; Coverage: 100%)
Gene names: LOC107767112, LOC107813756
Pfam: Ribosomal protein L6

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Name: 5S ribosomal RNA
Representative chains: C
Length: 119 nucleotides
Theoretical weight: 38.33 KDa
Rfam: 5S ribosomal RNA

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Name: 25S ribosomal RNA
Representative chains: A
Length: 3390 nucleotides
Theoretical weight: 1098.27 KDa
Rfam: Eukaryotic large subunit ribosomal RNA

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Name: 5.8S ribosomal RNA
Representative chains: B
Length: 163 nucleotides
Theoretical weight: 52.56 KDa
Rfam: 5.8S ribosomal RNA

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