› 8b9g

Electron Microscopy
2.86Å resolution

Cryo-EM structure of MLE in complex with ADP:AlF4 and U10 RNA

Released:
DOI: 10.2210/pdb8b9g/pdb
PDB entry 8b9g coloured by chain, front view.
PDB entry 8b9g coloured by chain, side view.
PDB entry 8b9g coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis of RNA-induced autoregulation of the DExH-type RNA helicase maleless.
Jagtap PKA, Müller M, Kiss AE, Thomae AW, Lapouge K, Beck M, Becker PB, Hennig J
Mol Cell 83 4318-4333.e10 (2023)
PMID: 37989319
Related structures: EMD-15931

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232451 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Dosage compensation regulator mle Chain: A
Molecule details ›
Chain: A
Length: 1159 amino acids
Theoretical weight: 130.47 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P24785 (Residues: 1-1159; Coverage: 90%)
Gene names: CG11680, mle, nap
Sequence domains:
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.02 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand ALF 1 x ALF
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.86Å
Relevant EMDB volumes: EMD-15931
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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